LncPrep + 96kb is a novel long non-coding RNA expressed in murine granulosa cells with two transcripts that are 2.2 and 2.8 kb in length. However, the potential roles of lncPrep + 96kb in granulosa cells remain poorly understood. In this study, we investigated the effect of the lncPrep + 96kb 2.2 kb transcript on granulosa cells through the overexpression and knockdown of lncPrep + 96kb 2.2 kb. We found that lncPrep + 96kb 2.2 kb inhibited aromatase expression and estradiol production. Endothelial differentiation-related factor 1 (EDF1) is an evolutionarily conserved transcriptional coactivator. We found that EDF1 knockdown inhibited aromatase expression and estradiol production. The RNA immunoprecipitation results also showed that lncPrep + 96kb 2.2 kb can bind to EDF1 and that overexpression of lncPrep + 96kb 2.2 kb induced the translocation of EDF1 from the nucleus to the cytoplasm. The CatRAPID signature revealed that the 1,979–2,077 and 603–690 nucleotide positions in lncPrep + 96kb 2.2 kb were potential binding sites for EDF1. We found that mutating the 1,979–2,077 site rescued the effects of lncPrep + 96kb 2.2 kb on aromatase expression and estradiol production. In conclusion, we are the first to report that specific expression of lncPrep + 96kb 2.2 kb in granulosa cells inhibits the production of estradiol by influencing the localization of EDF1 in granulosa cells. The 1,979–2,077 site of lncPrep + 96kb 2.2 kb contributes to the ability to bind to EDF1.

LncPrep + 96kb是在鼠颗粒细胞中表达的新型长非编码RNA，具有两个分别为2.2和2.8 kb的转录本。但是，仍不清楚lncPrep + 96kb在颗粒细胞中的潜在作用。在这项研究中，我们研究了lncPrep + 96kb 2.2 kb的过度表达和敲除对颗粒细胞的影响。我们发现lncPrep + 96kb 2.2 kb抑制了芳香化酶的表达和雌二醇的产生。内皮细胞分化相关因子1（EDF1）是进化上保守的转录共激活因子。我们发现EDF1组合式抑制芳香化酶表达和雌二醇的生产。RNA免疫沉淀结果还显示lncPrep + 96kb 2.2 kb可以与EDF1结合，并且lncPrep + 96kb 2的过表达。2 kb诱导EDF1从细胞核转移到细胞质。CatRAPID签名显示，lncPrep + 96kb 2.2 kb中的1,979–2,077和603–690核苷酸位置是EDF1的潜在结合位点。我们发现，突变1,979–2,077个位点可以挽救lncPrep + 96kb 2.2 kb对芳香化酶表达和雌二醇产生的影响。总之，我们是第一个报道lncPrep + 96kb 2.2 kb在颗粒细胞中的特异性表达，通过影响EDF1在颗粒细胞中的定位来抑制雌二醇的产生。lncPrep + 96kb 2.2 kb的1,979–2,077位点有助于与EDF1结合。我们发现，突变1,979–2,077个位点可以挽救lncPrep + 96kb 2.2 kb对芳香化酶表达和雌二醇产生的影响。总之，我们是第一个报道lncPrep + 96kb 2.2 kb在颗粒细胞中的特异性表达，通过影响EDF1在颗粒细胞中的定位来抑制雌二醇的产生。lncPrep + 96kb 2.2 kb的1,979–2,077位点有助于与EDF1结合。我们发现，突变1,979–2,077个位点可以挽救lncPrep + 96kb 2.2 kb对芳香化酶表达和雌二醇产生的影响。总之，我们是第一个报道lncPrep + 96kb 2.2 kb在颗粒细胞中的特异性表达，通过影响EDF1在颗粒细胞中的定位来抑制雌二醇的产生。lncPrep + 96kb 2.2 kb的1,979–2,077位点有助于与EDF1结合。