Abstract Genetic variability in date palm genotypes collected from different regions of southern Tunisia was analyzed using a Start Codon Targeted (SCoT) marker system. Thirty-one accessions collected from three locations were investigated. One hundred and nine amplicons were produced among which 84 % were polymorphic. The PIC value and the Rp values testified of the efficiency of used primers. The percentage of polymorphic loci (Pp) varied from 44.57 to 83.70 %, Nei‘s gene diversity (H) from 0.175 to 0.273 with a mean of 0.228, and Shannon‘s information index (I) values ranging from 0.257 to 0.409 with an average value of 0.338 were illustrated. The Tunisian date palm populations exhibited high genetic differentiation (GST = 0.319) and gene flow (Nm = 1.063). The AMOVA analysis presented 70 % of the variation within the population and 30 % of the variation between them. Phylogenetic analysis and the Bayesian clustering approach also revealed high genetic variation among genetic variants with a net divergence of the wild insular population of Kerkennah from other cultivars. The present investigation suggests the effectiveness of the SCoT marker system to estimate the genetic diversity of Phoenix dactylifera genotypes.

中文翻译：

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用于评估突尼斯 Phoenix dactylifera L. 栽培品种遗传多样性和种群结构的基因靶向标记

摘要 使用起始密码子靶向 (SCoT) 标记系统分析从突尼斯南部不同地区收集的枣椰树基因型的遗传变异性。调查了从三个地点收集的 31 份材料。产生了 109 个扩增子，其中 84% 是多态的。PIC 值和 Rp 值证明了所用引物的效率。多态位点百分比（Pp）从44.57%到83.70%不等，内氏基因多样性（H）从0.175到0.273，平均值为0.228，香农信息指数（I）值从0.257到0.409，平均值为0.38。被说明。突尼斯椰枣种群表现出高遗传分化（GST = 0.319）和基因流（Nm = 1.063）。AMOVA 分析显示了总体内 70% 的变异和它们之间 30% 的变异。系统发育分析和贝叶斯聚类方法还揭示了遗传变异之间的高遗传变异，Kerkennah 的野生岛屿种群与其他栽培品种之间存在净差异。目前的调查表明 SCoT 标记系统可以有效地估计 Phoenix dactylifera 基因型的遗传多样性。