Abstract The thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, thermal deactivation profiles of catalase Aspergillus niger were obtained at different temperatures in the range of 35°C to 70°C. It has been shown that the thermal deactivation of catalase Aspergillus niger follows the first-order model. The half-life time t1/2 of catalase Aspergillus niger at pH 7.0 and the temperature of 35°C and 70°C were 197 h and 1.3 h respectively. Additionally, t1/2 of catalase Aspergillus niger at the temperature of 5°C was calculated 58 months. Thermodynamic parameters the change in enthalpy ΔH*, the change in entropy ΔS* and the change Gibbs free energy ΔG* for the deactivation of catalase at different temperatures in the range of 35°C to 70°C were estimated. Catalase Aspergillus niger is predisposed to be used in biosensors by thermodynamics parameters obtained.

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过氧化氢酶黑曲霉热失活的热力学和动力学

摘要 基于酶的生物传感器的热稳定性对经济可行性至关重要。在这项研究中，过氧化氢酶黑曲霉的热失活曲线是在 35°C 到 70°C 范围内的不同温度下获得的。已经表明，过氧化氢酶黑曲霉的热失活遵循一阶模型。过氧化氢酶黑曲霉在 pH 7.0 和温度 35°C 和 70°C 下的半衰期 t1/2 分别为 197 小时和 1.3 小时。此外，在 5°C 温度下过氧化氢酶黑曲霉的 t1/2 计算为 58 个月。估算了在 35°C 至 70°C 范围内不同温度下过氧化氢酶失活的热力学参数，即焓变化 ΔH*、熵变化 ΔS* 和吉布斯自由能变化 ΔG*。