Our laboratory and others have use radiation metabolomics to assess responses in order to develop biomarkers reflecting exposure and level of injury. To expand the types of exposure and compare to previously published results, metabolomic analysis has been carried out using serum samples from mice exposed to ¹³⁷Cs internal emitters. Animals were injected intraperitoneally with ¹³⁷CsCl solutions of varying radioactivity, and the absorbed doses were calculated. To determine the dose rate effect, serum samples were collected at 2, 3, 5, 7, and 14 days after injection. Based on the time for each group receiving the cumulative dose of 4 Gy, the dose rate for each group was determined. The dose rates analyzed were 0.16 Gy/day (low), 0.69 Gy/day (medium), and 1.25 Gy/day (high). The results indicated that at a cumulative dose of 4 Gy, the low dose rate group had the least number of statistically significantly differential spectral features. Some identified metabolites showed common changes for different dose rates. For example, significantly altered levels of oleamide and sphingosine 1-phosphate were seen in all three groups. On the other hand, the intensity of three amino acids, Isoleucine, Phenylalanine and Arginine, significantly decreased only in the medium dose rate group. These findings have the potential to be used in assessing the exposure and the biological effects of internal emitters.

中文翻译：

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与不同剂量率的铯137内部发射体相关的血清代谢组学改变。

我们的实验室和其他实验室已经使用辐射代谢组学来评估反应，从而开发出反映暴露程度和伤害水平的生物标志物。为了扩大暴露的类型并与以前发表的结果进行比较，使用暴露于¹³⁷ Cs内部发射源的小鼠的血清样品进行了代谢组学分析。给动物腹膜内注射¹³⁷计算出不同放射性的CsCl溶液和吸收剂量。为了确定剂量率效应，在注射后第2、3、5、7和14天收集血清样品。基于每组接受4 Gy累积剂量的时间，确定每组的剂量率。分析的剂量率是0.16 Gy /天（低），0.69 Gy /天（中）和1.25 Gy /天（高）。结果表明，在累积剂量为4 Gy的情况下，低剂量率组的统计学差异显着。一些鉴定出的代谢物显示出不同剂量率的共同变化。例如，在所有三组中均观察到油酰胺和1-磷酸鞘氨醇水平的显着改变。另一方面，异亮氨酸，苯丙氨酸和精氨酸这三种氨基酸的强度 仅在中等剂量率组中显着降低。这些发现有可能用于评估内部辐射源的暴露和生物学效应。