Malaria is a major global health problem, where the anautogenous female mosquito Anopheles gambiae serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less than 20% of all small ncRNAs in our small RNA-Seq libraries. To this end, we systematically classified multiple small ncRNA groups across mosquito tissues. Here we (i) determined a new enriched-midgut miRNA, (ii) updated the piRNA annotation in ovaries with a genomic map of unique-mapping piRNAs, (iii) identified pan-tissue and tissue-enriched mRNA-derived small ncRNAs, and (iv) assessed AGO1- and AGO2- loading of candidate small ncRNAs. Continued research will broaden our view of small ncRNAs and greatly aide in our understanding on how these molecules contribute to mosquito physiology.

中文翻译：

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疟疾媒介冈比亚按蚊组织中小非编码 RNA 群体的全球分析。


疟疾是一个主要的全球健康问题，其中自生雌性冈比亚按蚊是主要媒介。为了对抗这种毁灭性的疾病，了解蚊子的生理学至关重要。载体领域的大量研究表明，小非编码 RNA (ncRNA) 在蚊子生理学的许多方面发挥着重要作用。虽然我们之前的 miRNA 注释工作证明了不同组织中的表达动态，但在我们的小 RNA-Seq 文库中，miRNA 只占所有小 ncRNA 的不到 20%。为此，我们系统地对蚊子组织中的多个小 ncRNA 群体进行了分类。在这里，我们 (i) 确定了一种新的富集中肠 miRNA，(ii) 使用独特映射 piRNA 的基因组图更新了卵巢中的 piRNA 注释，(iii) 鉴定了泛组织和组织富集的 mRNA 衍生的小 ncRNA，以及(iv) 评估候选小 ncRNA 的 AGO1 和 AGO2 负载。持续的研究将拓宽我们对小 ncRNA 的认识，并极大地帮助我们了解这些分子如何促进蚊子的生理学。