Dietary carbohydrates are anaerobically fermented by gut microbiota to short-chain fatty acids (SCFAs), conferring gut health benefits. Of all tested prebiotics, galactooligosaccharides (GOS) and resistant starch (RS) stimulated the SCFA production in mud crab (Scylla paramamosain), a crustacean model, to a greater extent than the other carbohydrates tested. Using in vitro anaerobic fermentation cultures, this study further explored the prebiotic potential of GOS and RS in mud crab by assessing their impacts on gut microbiota changes and SCFA production. Both GOS and RS significantly promoted SCFA production. Bacterial diversity in the GOS group was lower than in the RS or control group. GOS promoted the growth of Bacteroidetes, while RS promoted Tenericutes. A strong positive correlation was found between SCFA production and bacterial abundance; most bacteria per se correlated with each other. The findings demonstrated the prebiotic potential of GOS and RS in mud crab.

饮食中的碳水化合物被肠道菌群厌氧发酵为短链脂肪酸（SCFA），赋予肠道健康益处。在所有测试的益生元中，低聚半乳糖（GOS）和抗性淀粉（RS）刺激了蟹蟹的SCFA产生（拟南芥），一种甲壳动物模型，其程度要比其他受测试的碳水化合物高。使用体外通过厌氧发酵培养，本研究通过评估它们对肠道微生物群变化和SCFA产生的影响，进一步探索了GOS和RS在泥蟹中的益生元潜力。GOS和RS都大大促进了SCFA的生产。GOS组的细菌多样性低于RS或对照组。GOS促进了拟杆菌的生长，而RS促进了Tenericutes。发现SCFA的产生与细菌丰度之间存在很强的正相关性。大多数细菌本身相互关联。这些发现证明了GOS和RS在泥蟹中的益生元潜力。