To investigate the mechanisms of phospholipase C (PLC)-mediated calcium (Ca²⁺) signaling in Alternaria alternata, the regulatory roles of PLC were elucidated using neomycin, a specific inhibitor of PLC activity. Three isotypes of PLC designated AaPLC1, AaPLC2, and AaPLC3 were identified in A. alternata through genome sequencing. qRT-PCR analysis showed that fruit wax extracts significantly upregulated the expression of all three PLC genes in vitro. Pharmacological experiments showed that neomycin treatment led to a dose-dependent reduction in spore germination and appressorium formation in A. alternata. Appressorium formation was stimulated on hydrophobic and pear wax-coated surfaces but was significantly inhibited by neomycin treatment. The appressorium formation rates of neomycin treated A. alternata on hydrophobic and wax-coated surfaces decreased by 86.6 and 47.4%, respectively. After 4 h of treatment, exogenous CaCl₂ could partially reverse the effects of neomycin treatment. Neomycin also affected mycotoxin production in alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), and tentoxin (TEN), with exogenous Ca²⁺ partially reversing these effects. These results suggest that PLC is required for the growth, infection structure differentiation, and secondary metabolism of A. alternata in response to physiochemical signals on the pear fruit surface.

调查磷脂酶C（PLC）介导的钙（Ca ²⁺）信号转导的机制。链格孢，使用新霉素（一种新的PLC活性抑制剂）阐明了PLC的调节作用。指定了PLC的三种同型AaPLC1， AaPLC2和 AaPLC3 在 交链孢霉通过基因组测序。qRT-PCR分析表明，水果蜡提取物显着上调了所有三个PLC基因的表达体外。药理实验表明，新霉素处理可导致孢子萌发和app的形成呈剂量依赖性减少。交链孢霉。在疏水和梨蜡涂层的表面上刺激了Appressorium的形成，但新霉素处理显着抑制了Appressorium的形成。新霉素处理后的前ress形成率交链孢霉疏水表面和蜡涂层表面的表面活性剂分别减少了86.6％和47.4％。处理4小时后，外源CaCl ₂可以部分逆转新霉素治疗的作用。新霉素还影响了交替糖（AOH），交替糖单甲醚（AME），altenuene（ALT）和Tentoxin（TEN）中霉菌毒素的产生，外源Ca ^2+可以部分逆转这些作用。这些结果表明PLC是生长，感染结构分化和次级代谢所必需的交链孢霉 响应梨果实表面的生理化学信号。