The study was designed to decipher the inter‐relationship between peripheral hormones (kisspeptin and testosterone), sexual behaviour and seminal variables of Murrah buffalo bulls (n = 134). In this study, we recorded that 13%, 37%, 40%, 6% and 4% Murrah buffalo bulls had reaction time of <30, 31–60, 61–180, 181–300 and >300 s, respectively. Further, it was observed that 4%, 85% and 10% buffalo bulls were sexually aggressive, active and dull, respectively, during semen collection. The courtship behaviour was not found to be desirable for the bulls used for the semen collection. Mean of ejaculate volume, sperm concentration and mass motility (0–5 scale) were 3.57 ml, 977.11 million/ml, 2.7, respectively. Correlation studies revealed that the reaction time was positively correlated with courtship behaviour and body weight, and negatively correlated with sexual aggressiveness and sperm concentration. Serum kisspeptin in buffalo bulls, measured for the first time, was found to 3.8 ± 0.7 ng/ml. Serum kisspeptin and testosterone level are negatively correlated to each other and kisspeptin level influenced the sexual behaviour (reaction time, sexual aggressiveness and penile erection) of study bulls. Serum kisspeptin was higher in the buffalo bulls with higher sperm concentration indicating its role in spermatogenesis. In conclusion, for the first time basic information related to sexual behaviour of Murrah buffalo bulls in large population along with its inter‐relationship with peripheral hormones (kisspeptin and testosterone) has been documented.

中文翻译：

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血清Kisspeptin：水牛公牛性行为和精子浓度的新可能生物标记。

所述研究被设计为解密的外周激素（亲吻促动素和睾酮），性行为和摩拉水牛公牛精液的变量之间的相互关系（Ñ = 134）。在这项研究中，我们记录了13％，37％，40％，6％和4％的Murrah水牛公牛的反应时间分别小于30、31-60、61-180、181-300和> 300 s。此外，观察到在收集精液期间，分别有4％，85％和10％的水牛公牛具有性侵害性，活跃性和钝性。对于用于收集精液的公牛，人们并不认为求偶行为是理想的。平均射精量，精子浓度和运动能力（0-5级）分别为3.57 ml，977.11百万/ml、2.7。相关性研究表明，反应时间与求爱行为和体重呈正相关，与性侵略性和精子浓度呈负相关。水牛公牛的血清kisseptin首次测量为3.8±0.7 ng / ml。血清基肽素和睾丸激素水平彼此负相关，而基肽素水平影响研究公牛的性行为（反应时间，性侵略性和阴茎勃起）。在水牛公牛中，血清kisepteptin较高，精子浓度较高，表明其在精子发生中的作用。总之，首次记录了与人口众多的穆拉水牛公牛的性行为有关的基本信息，以及与外围激素（毒肽和睾丸激素）的相互关系。