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Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent
Luminescence ( IF 3.2 ) Pub Date : 2020-06-30 , DOI: 10.1002/bio.3908 Yue Zhang 1 , Gaoxi Cui 1 , Yuchan Meng 1 , Yujing Wang 1 , Xu Hun 1
Luminescence ( IF 3.2 ) Pub Date : 2020-06-30 , DOI: 10.1002/bio.3908 Yue Zhang 1 , Gaoxi Cui 1 , Yuchan Meng 1 , Yujing Wang 1 , Xu Hun 1
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Bacterial pathogen control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) was synthesized using the oleylamine reducing method. It was found that Cu/Co/Ni TNA greatly enhanced the chemiluminescence (CL) signal of the hydroxylamine‐O‐sulfonic acid (HOSA)–luminol system. The CL properties of Cu/Co/Ni TNA were investigated systemically. The possible CL mechanism also was intensively investigated. Based on the enhanced CL phenomenon of Cu/Co/Ni TNA, a Cu/Co/Ni TNA, penicillin, and anti‐L. monocytogenes (Listeria monocytogenes) antibody‐based sandwich complex assay for detection of L. monocytogenes was established. In this sandwich CL assay, penicillin was employed to capture and enrich pathogenic bacteria with penicillin‐binding proteins (PBPs) while anti‐L. monocytogenes antibody was adopted as the specific recognition molecule to recognize L. monocytogenes. L. monocytogenes was detected sensitively based on this new Cu/Co/Ni TNA–HOSA–luminol CL system. The CL intensity was proportional to the L. monocytogenes concentration ranging from 2.0 × 102 CFU ml−1 to 3.0 × 107 CFU ml−1 and the limit of detection wa 70 CFU ml−1. The reliability and potential applications of our method was verified by comparison with official methods and recovery tests in environment and food samples.
中文翻译：
基于铜/钴/镍三元纳米催化剂结合青霉素作为通用捕获剂的单核细胞增生李斯特菌化学发光分析
细菌病原体控制在海鲜生产中很重要。在这项研究中，使用油胺还原法合成了Cu / Co / Ni三元纳米合金（Cu / Co / Ni TNA）。发现Cu / Co / Ni TNA大大增强了羟胺-O-磺酸（HOSA）-鲁米诺系统的化学发光（CL）信号。系统地研究了Cu / Co / Ni TNA的CL性质。还对可能的CL机制进行了深入研究。基于Cu / Co / Ni TNA的增强CL现象，Cu / Co / Ni TNA，青霉素和抗L物质。单核细胞（单增李斯特菌），用于检测的基于抗体的夹心复合物测定大号。单核细胞增生建立了。在此夹心式CL试验中，青霉素被用于捕获和富集具有抗L的青霉素结合蛋白（PBP）的致病细菌。单核细胞增生因子抗体被用作识别L的特异性识别分子。单核细胞增生。大号。基于这种新的Cu / Co / Ni TNA-HOSA-luminol CL系统灵敏地检测到单核细胞增生。CL强度与L成正比。单核细胞增生李斯特菌浓度范围为2.0×10 2 CFU ml -1至3.0×10 7 CFU ml -1，检出限为70 CFU ml-1。通过与官方方法进行比较以及在环境和食品样品中进行回收率测试，验证了我们方法的可靠性和潜在应用性。
更新日期：2020-06-30
中文翻译：
基于铜/钴/镍三元纳米催化剂结合青霉素作为通用捕获剂的单核细胞增生李斯特菌化学发光分析
细菌病原体控制在海鲜生产中很重要。在这项研究中，使用油胺还原法合成了Cu / Co / Ni三元纳米合金（Cu / Co / Ni TNA）。发现Cu / Co / Ni TNA大大增强了羟胺-O-磺酸（HOSA）-鲁米诺系统的化学发光（CL）信号。系统地研究了Cu / Co / Ni TNA的CL性质。还对可能的CL机制进行了深入研究。基于Cu / Co / Ni TNA的增强CL现象，Cu / Co / Ni TNA，青霉素和抗L物质。单核细胞（单增李斯特菌），用于检测的基于抗体的夹心复合物测定大号。单核细胞增生建立了。在此夹心式CL试验中，青霉素被用于捕获和富集具有抗L的青霉素结合蛋白（PBP）的致病细菌。单核细胞增生因子抗体被用作识别L的特异性识别分子。单核细胞增生。大号。基于这种新的Cu / Co / Ni TNA-HOSA-luminol CL系统灵敏地检测到单核细胞增生。CL强度与L成正比。单核细胞增生李斯特菌浓度范围为2.0×10 2 CFU ml -1至3.0×10 7 CFU ml -1，检出限为70 CFU ml-1。通过与官方方法进行比较以及在环境和食品样品中进行回收率测试，验证了我们方法的可靠性和潜在应用性。
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