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A reactive probe for Co2+ ion detection based on a catalytic decomposition process and its fluorescence imaging in living cells
Luminescence ( IF 3.2 ) Pub Date : 2020-06-30 , DOI: 10.1002/bio.3909 Liguo Ji 1 , Chengliang Yang 1 , Huijie Li 1 , Nan Yang 1 , Yutian Fu 1 , Linlin Yang 1 , Qingzhi Wang 1 , Guangjie He 1
Luminescence ( IF 3.2 ) Pub Date : 2020-06-30 , DOI: 10.1002/bio.3909 Liguo Ji 1 , Chengliang Yang 1 , Huijie Li 1 , Nan Yang 1 , Yutian Fu 1 , Linlin Yang 1 , Qingzhi Wang 1 , Guangjie He 1
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A novel reactive fluorescent probe for cobalt ions was prepared based on integration of thiourea functional groups, coumarin, and naphthalimide fluorophores. There was no fluorescence observed for the probe itself, however, in the presence of cobalt ions, catalytic decomposition occurred for the probe and coumarin molecular fragments were produced that emitted blue fluorescence. This enabled the probe to be used as a ‘turn on’ reagent for detection of cobalt ions. Under physiological pH conditions and in appropriate solvent systems, an obvious fluorescence enhancement for cobalt ions was observed in selective experiments. Competition experiments indicated that cobalt ions could still induce fluorescence enhancement in the presence of other metal ions. Sensitivity experiments showed that the detection limit for cobalt ions was 6.0 nM. Dynamics research demonstrated that the catalytic process was a pseudo‐first‐order reaction and the reaction constant (kobs) was calculated to be 1.49 × 10−2 min−1. In addition, the mechanism of catalytic decomposition could be demonstrated using electrospray ionization mass spectrometry and thin layer chromatography experiments. Cell fluorescence imaging experiments demonstrated that the probe could be used to detect cobalt ions in living HeLa cells.
中文翻译：
基于催化分解过程的Co2 +离子检测反应探针及其在活细胞中的荧光成像
基于硫脲官能团，香豆素和萘二甲酰亚胺荧光团的整合，制备了一种新型的钴离子反应性荧光探针。没有观察到探针本身的荧光，但是，在存在钴离子的情况下，探针发生了催化分解，并且产生了香豆素分子片段，发出了蓝色荧光。这使该探针可用作检测钴离子的“开启”试剂。在生理pH条件下和适当的溶剂体系中，选择性实验观察到钴离子的荧光增强明显。竞争实验表明，在其他金属离子存在下，钴离子仍然可以诱导荧光增强。灵敏度实验表明，钴离子的检出限为6.0 nM。obs）计算为1.49×10 -2 min -1。此外，可以使用电喷雾电离质谱和薄层色谱实验来证明催化分解的机理。细胞荧光成像实验表明该探针可用于检测活HeLa细胞中的钴离子。
更新日期：2020-06-30
中文翻译：
基于催化分解过程的Co2 +离子检测反应探针及其在活细胞中的荧光成像
基于硫脲官能团，香豆素和萘二甲酰亚胺荧光团的整合，制备了一种新型的钴离子反应性荧光探针。没有观察到探针本身的荧光，但是，在存在钴离子的情况下，探针发生了催化分解，并且产生了香豆素分子片段，发出了蓝色荧光。这使该探针可用作检测钴离子的“开启”试剂。在生理pH条件下和适当的溶剂体系中，选择性实验观察到钴离子的荧光增强明显。竞争实验表明，在其他金属离子存在下，钴离子仍然可以诱导荧光增强。灵敏度实验表明，钴离子的检出限为6.0 nM。obs）计算为1.49×10 -2 min -1。此外，可以使用电喷雾电离质谱和薄层色谱实验来证明催化分解的机理。细胞荧光成像实验表明该探针可用于检测活HeLa细胞中的钴离子。
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