The aim of this study was to select and identify thermophilic bacteria from Caatinga biome (Brazil) able to produce thermoactive keratinases and characterize the keratinase produced by the selected isolate. After enrichment in keratin culture media, an Anoxybacillus caldiproteolyticus PC2 was isolated. This thermotolerant isolate presents a remarkable feature producing a thermostable keratinase at 60°C. The partially purified keratinase, identified as a thermolysin‐like peptidase, was active at a pH range of 5.0–10.0 with maximal activity at a temperature range of 50–80°C. The optimal activity was observed at pH 7.0 and 50–60°C. These characteristics are potentially useful for biotechnological purposes such as processing and bioconversion of keratin.

中文翻译：

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嗜极微生物 Anoxybacillus sp.从巴西半干旱地区（卡廷加）分离的 PC2 产生耐热角蛋白酶


本研究的目的是选择和鉴定来自卡廷加生物群落（巴西）的能够产生热活性角蛋白酶的嗜热细菌，并表征所选分离株产生的角蛋白酶。在角蛋白培养基中富集后，分离出解热厌氧杆菌 PC2。这种耐热分离物具有显着的特征，可在 60°C 下产生热稳定性角蛋白酶。部分纯化的角蛋白酶被鉴定为嗜热菌蛋白酶样肽酶，在 5.0-10.0 的 pH 范围内具有活性，在 50-80°C 的温度范围内具有最大活性。在 pH 7.0 和 50–60°C 下观察到最佳活性。这些特性对于生物技术目的可能有用，例如角蛋白的加工和生物转化。