PCR‐sequence‐specific oligonucleotide (PCR‐SSO) is commonly used for HLA‐typing. We recently replaced LabType SSO HD kit for HLA‐C typing with the XR kit (OneLambda, Inc.). We used 137 patients' samples representing unique most likely HLA‐C allele combinations to compare these kits' performance. The XR kit decreased the number of allele ambiguities with a median of 26.1% (first to third quartiles 20% to 36.2%, range 0% to 96.1%). The XR kit did not resolve all the common, intermediate and well‐documented HLA allele ambiguities, which may be important in the clinical setting. The XR kit eliminated 23.6% of null allele ambiguities. In conclusion, the HLA‐C XR kit provides a moderate yet valuable improvement of HLA‐typing resolution in comparison with the HLA‐C HD kit.

中文翻译：

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新的序列特异性寡核苷酸试剂盒可改善HLA-C分型。

PCR序列特异性寡核苷酸（PCR-SSO）通常用于HLA分型。我们最近用XR套件（OneLambda，Inc.）替换了用于HLA-C输入的LabType SSO HD套件。我们使用代表独特的最可能的HLA-C等位基因组合的137位患者样品来比较这些试剂盒的性能。XR试剂盒减少了等位基因歧义的数量，中位数为26.1％（第一至第三四分位数为20％至36.2％，范围为0％至96.1％）。XR试剂盒不能解决所有常见的，中间的和有据可查的HLA等位基因的歧义，这在临床环境中可能很重要。XR试剂盒消除了23.6％的无效等位基因歧义。总之，与HLA-C HD套件相比，HLA-C XR套件在HLA分型分辨率方面提供了适度但有价值的改进。