Composite hydrogels based on natural polymers arouse interest as sustainable matrices for the delivery of bioactive materials. This study investigates the possibility of achieving high thermally and hydrolytically stable, well-defined microstructure bi-polymer composite hydrogels using only natural materials at a low energy process. Firstly, citric acid (CA) crosslinked hybrid hydrogel matrices are developed by varying mole ratio of carboxymethyl cellulose (CMC) to chitosan (CSN) by 1:1, 1:2 and 2:1. Then, hydrolytic, thermal and structural properties of the matrices are studied to determine microstructure. Lastly, the matrices are functionalized with beta-cyclodextrin (β-CD), and the effect of β-CD on hydrogels' microstructure and antibacterial activity is examined. Optimum microstructure is found at CMC:CSN = 1:1 for CMC~CA~CSN with a gel fraction of 63%, which increases to 82% when reinforced with β-CD. Interestingly, CMC~CA~2CSN exhibits a combination of good crosslinking, super-absorbency (1229.7% water absorbency and 2200% swelling) yet enhanced hydrolytic stability (84.7% gel fraction). Complexation with β-CD propagates the growth of Gram-positive bacterium, Corynebacterium glutamicum ATCC 13032. Overall, CMC~CA~CSN~β-CD is considered as a green, crystalline, thermally and hydrolytically stable composite hydrogel matrix.

基于天然聚合物的复合水凝胶引起了人们对于作为生物活性物质输送的可持续基质的兴趣。这项研究调查了仅使用天然材料在低能量过程中获得高热和水解稳定，定义明确的微结构双聚合物复合水凝胶的可能性。首先，通过将羧甲基纤维素（CMC）与壳聚糖（CSN）的摩尔比更改为1：1、1：2和2：1，开发出柠檬酸（CA）交联杂化水凝胶基质。然后，研究基质的水解，热和结构性质，以确定其微观结构。最后，将基质用β-环糊精（β-CD）功能化，并检查β-CD对水凝胶微结构和抗菌活性的影响。在CMC：CSN = 1处找到最佳的微观结构：CMC〜CA〜CSN为1，凝胶分数为63％，当用β-CD增强时，凝胶分数增加至82％。有趣的是，CMC〜CA〜2CSN具有良好的交联性，高吸收性（1229.7％的吸水率和2200％的溶胀性）以及增强的水解稳定性（84.7％的凝胶分数）的组合。与β-CD的复合作用可促进革兰氏阳性细菌的生长，谷氨酸棒杆菌ATCC13032。总的来说，CMC〜CA〜CSN〜β-CD被认为是绿色，结晶，热和水解稳定的复合水凝胶基质。