FGF-2 accumulates in many tumor tissues and is closely related to the development of tumor angiogenesis and the immunosuppressive microenvironment. This study aimed to investigate whether active immunization against FGF-2 could modify antitumor immunity and enhance the efficacy of an HPV16 E7-specific therapeutic vaccine. Combined immunization targeting both FGF-2 and E7 significantly suppressed tumor growth, which was accompanied by significantly increased levels of IFN-γ-expressing splenocytes and effector CD8 T cells and decreased levels of immunosuppressive cells such as regulatory T cells (Tregs) and myeloid-derived suppressor cells(MDSCs) in both the spleen and tumor; in addition, the levels of FGF-2 and neovascularization in tumors were decreased in the mice receiving the combined immunization, and tumor cell apoptosis was promoted. The combination of an HPV16 E7-specific vaccine and active immunization against FGF-2 significantly enhances antitumor immune responses in mice with TC-1 tumors, indicating a promising strategy for tumor immunotherapy.

FGF-2积累在许多肿瘤组织中，与肿瘤血管生成的发展和免疫抑制微环境密切相关。这项研究旨在调查针对FGF-2的主动免疫是否可以修饰抗肿瘤免疫力并增强HPV16 E7特异性治疗疫苗的功效。针对FGF-2和E7的联合免疫可显着抑制肿瘤的生长，同时伴随着表达IFN-γ的脾细胞和效应CD8 T细胞水平的显着增加以及免疫抑制细胞（例如调节性T细胞（Tregs）和髓样-脾和肿瘤中衍生的抑制细胞（MDSCs）；此外，接受联合免疫的小鼠的肿瘤中FGF-2和新血管形成水平降低，并促进了肿瘤细胞凋亡。