Like mammalian cells, helminth parasites are equipped with an array of enzymatic anti-oxidant system which has an adaptive strategy to cope up with several conditions of stress that arise from host immune response or drug treatment. Earlier, we had reported that three species of Senna, viz. S. alata, S. alexandrina and S. occidentalis leaf extracts caused severe morphological and biochemical alterations in the zoonotic parasite Hymenolepis diminuta. To understand whether the leaf extracts of the three species of Senna have any effect on the enzymatic anti-oxidant system in H.diminuta or not, the present study was investigated on the mechanism of action of these leaf extracts on the anti-oxidant system of the parasite. The viability of the parasite was assessed by MTT reduction assay, chromatin condensation through Hoechst staining of tissue and DNA fragmentation assay, and the oxidative enzymes of the parasite were estimated biochemically. Activity of superoxide dismutase, catalase, glutathione S- transferase and glutathione peroxidase were found to be increased in all the treated parasites from that of the control, with S. alata showed the highest increased amongst the three plant species in all the enzymes, at 331.0 %, 215.4 %, 85.4 % and 65.5 % respectively. Upliftment of apoptotic protein CED-3, CED-4 and EGL-1 and down regulation of anti-apototic protein CED-9 was visualised in all treated paraites. The redox imbalance triggered by these leaf extracts resulted in the activation of apoptotic pathway that led to death of the parasite. Our results demonstrated that the leaf extracts of the three Senna plant species could open new insight for an affordable natural anthelmintic with high efficacy and less toxicity.

像哺乳动物细胞一样，蠕虫寄生虫也配备了一系列酶促抗氧化剂系统，该系统具有适应性策略，可以应对宿主免疫反应或药物治疗引起的多种压力条件。之前，我们曾报道过塞纳（Senna）的3种。S. alata，S。alexandrina和S. occidentalis叶提取物在人畜共患寄生虫Hymenolepis diminuta中引起了严重的形态和生化变化。了解三种番泻叶提取物是否对H.diminuta的酶促抗氧化系统有影响是否研究本叶提取物对寄生虫抗氧化系统的作用机理进行了研究。通过MTT还原测定，通过组织的Hoechst染色和DNA片段化测定的染色质浓缩，以及通过生化评估该寄生虫的氧化酶，评估了该寄生虫的生存力。发现所有处理过的寄生虫的超氧化物歧化酶，过氧化氢酶，谷胱甘肽S-转移酶和谷胱甘肽过氧化物酶的活性均比对照高。在所有酶中，三种植物的增幅最高，分别为331.0％，215.4％，85.4％和65.5％。在所有处理过的寄生虫中均可见凋亡蛋白CED-3，CED-4和EGL-1的增强和抗凋亡蛋白CED-9的下调。这些叶提取物引发的氧化还原失衡导致凋亡途径的激活，导致寄生虫死亡。我们的结果表明，三种番泻叶植物的叶提取物可以为负担得起的天然驱虫药打开新的视野，该药具有较高的功效和较低的毒性。