Flow cytometry (FCM) is a powerful technique still little used to study filamentous fungi due to physical constraints: the hyphae are too large to enter the FCM fluidic system, unless spores can be analyzed at a very early stage of germination. The technique nevertheless has strong potential for the study of these micro-organisms (spore sorting, viability, characterization etc.). This study focused on the investigation of several parameters, ranging from germination and storage conditions of T. reesei spores through to FCM gating, to detect their fluorescence during the first 24 h of germination. Fluorescent spores were first obtained after aerobic germination at 25 °C and monitored over 24 h using FCM, to screen for nine promoters controlling a green fluorescent protein gene. The fluorescence signal (FL1) was then acquired, in addition to the growth characterization of the spores, based on the size signal or Forward Scatter (FSC). They were combined to identify the best candidate(s) from among the nine promoters for the strongest- and earliest-possible fluorescence emission, which resulted in the following ranking: pTEF > pPKI > pGPD > pPDC. There are numerous possible applications of this work, ranging from molecular biology to monitoring fermentation.

流式细胞术（FCM）是一项功能强大的技术，由于物理限制，它仍很少用于研究丝状真菌：除非菌丝在萌芽的早期就可以进行分析，否则菌丝太大而无法进入FCM流体系统。尽管如此，该技术仍具有研究这些微生物的强大潜力（孢子分选，生存力，特性等）。这项研究着重于研究几个参数，从里氏木霉的发芽和贮藏条件孢子进入FCM门控，以在发芽的最初24小时内检测其荧光。在25°C有氧萌发后，首先获得荧光孢子，并使用FCM监测24小时，以筛选控制绿色荧光蛋白基因的9个启动子。然后，除了基于孢子的生长特征，还基于大小信号或前向散射（FSC）获取荧光信号（FL1）。将它们组合在一起，从最强和最早可能的荧光发射的9个启动子中找出最佳候选者，从而得出以下排名：pTEF> pPKI> pGPD> pPDC。这项工作有许多可能的应用，从分子生物学到监测发酵。