Background

The nonlesional skin of children with atopic dermatitis (AD) with peanut allergy (PA) is associated with increased transepidermal water loss; low urocanic acid (UCA) and pyrrolidone carboxylic acid (PCA), both of which are filaggrin breakdown products; and a reduced ratio of esterified ω-hydroxy fatty acid sphingosine ceramides (EOS-CERs) to nonhydroxy fatty acid sphingosine ceramides (NS-CERs) in the skin. The skin barrier of subjects with PA without AD (AD^–PA⁺) has not been studied.

Objective

Our aim was to explore whether AD^–PA⁺ is associated with skin barrier abnormalities.

Methods

A total of 33 participants were enrolled, including 13 AD^–PA⁺, 9 AD⁺PA⁺, and 11 nonatopic (NA) participants.

Results

The PCA content in the stratum corneum of AD^–PA⁺ subjects was significantly reduced versus that in NA subjects (median level, 67 vs 97 μg/mg protein [P = .028]). The ratio between cis- and trans-UCA decreased significantly from being highest in the NA group (1.62) to lowest in AD⁺PA⁺ group (0.07 [P < .001 vs in the NA group; P = .006 vs in the AD^–PA⁺ group]), with the AD^–PA⁺ group having an intermediate cis/trans-UCA ratio (1.17 [P = .024 vs in the NA group]). The TEWL in AD^–PA⁺ subjects did not differ from that in the group with NA skin. Interestingly, AD^–PA⁺ subjects had an increased EOS/NS-CER ratio versus that in the group of subjects with NA skin (1.9 vs 1.3 [P = .008]), whereas the AD⁺PA⁺ group had a decreased proportion of EOS-CERs (0.8 [P = .001] vs in the AD^–PA⁺ group).

Conclusion

Our data demonstrate that irrespective of AD, PA is associated with decreased skin cis-UCA and PCA content. An increase in skin EOS-CER/NS-CER ratio separates the AD^–PA⁺ group from the AD⁺PA⁺ and NA groups.

中文翻译：

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花生过敏但无特应性皮炎患者的独特皮肤异常。

背景

患有花生过敏 (PA) 的特应性皮炎 (AD) 儿童的无损伤皮肤与经表皮水分流失增加有关；低尿刊酸（UCA）和吡咯烷酮羧酸（PCA），两者都是丝聚蛋白分解产物；以及皮肤中酯化的 ω-羟基脂肪酸鞘氨醇神经酰胺 (EOS-CER) 与非羟基脂肪酸鞘氨醇神经酰胺 (NS-CER) 的比例降低。没有研究过没有 AD 的 PA 受试者的皮肤屏障 (AD ^– PA ⁺ )。

客观的

我们的目的是探索 AD ^– PA ⁺是否与皮肤屏障异常有关。

方法

共招募了 33 名参与者，包括 13 名 AD ^– PA ⁺、9 名 AD ⁺ PA ⁺和 11 名非特应性 (NA) 参与者。

结果

^{AD –} PA ⁺受试者角质层中的 PCA 含量与 NA 受试者相比显着降低（中位数水平，67 对 97 μg/mg 蛋白质 [ P  = .028]）。顺式-反式-UCA之间的比率从 NA 组的最高值 (1.62) 显着下降到 AD ⁺ PA ⁺组的最低值 (0.07 [ P  < .001 vs NA 组；P  = .006 vs AD ^– PA ⁺ group]），AD ^– PA ⁺ group 具有中等的顺式/反式-UCA 比率（1.17 [ P = .024 vs NA 组]）。^{AD –} PA ⁺受试者的 TEWL与 NA 皮肤组没有差异。有趣的是，AD ^- PA ⁺受试者的 EOS/NS-CER 比率与 NA 皮肤受试者组相比有所增加（1.9 vs 1.3 [ P  = .008]），而 AD ⁺ PA ⁺组的比例降低EOS-CER（0.8 [ P  = .001] 与 AD ^– PA ⁺组相比）。

结论

我们的数据表明，与 AD 无关，PA 与皮肤 cis-UCA 和 PCA 含量降低有关。皮肤 EOS-CER/NS-CER 比率的增加将 AD ^– PA ⁺组与 AD ⁺ PA ⁺和 NA 组分开。