Abstract This study investigated the effect of salinity on ammonia-oxidizing archaea and bacteria (AOA and AOB) by operating two bioreactors under the salinity level of 2.5‰ and 35‰, respectively. Both bioreactors achieved more than 95% of nitrification efficiency, but distinct AOA and AOB populations were enriched in the two bioreactors. For AOB, Nitrosomonas marina-like AOB and Nitrosomonas oligotropha-like AOB were dominated in high- and low-salinity bioreactors, respectively, while two different AOAs in marine group were dominated in high- and low-salinity bioreactors, respectively. The results from the amoA gene based real-time quantitative polymerase chain reaction indicated that, after 150 days of operation, AOA was more abundant than AOB by 1.6–54 and 2.3–81.1 times in the high and low salinity bioreactors, respectively. The results of inhibitory tests for seven chemicals on AOA-enriched sludge showed that 0.5 mg/L of phenol, benzene, toluene, and pyridine caused more than 55% of nitrification inhibition on AOA-enriched sludge. In comparison with AOB, AOA, however, showed higher tolerance to dimethyl sulfide and allylthiourea. Ethylenediamine presented a similar inhibitory effect on AOA and AOB.

中文翻译：

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高盐度和低盐度下富集氨氧化古菌的丰度、群落结构和硝化抑制作用

摘要 本研究通过在盐度分别为2.5‰和35‰的条件下运行两个生物反应器，研究了盐度对氨氧化古菌和细菌（AOA和AOB）的影响。两种生物反应器的硝化效率都达到了 95% 以上，但两种生物反应器中富含不同的 AOA 和 AOB 种群。对于AOB，Nitrosomonas marina-like AOB和Nitrosomonas oligotropha-like AOB分别在高盐度和低盐度生物反应器中占主导地位，而海洋组中两种不同的AOA分别在高盐度和低盐度生物反应器中占主导地位。基于 amoA 基因的实时定量聚合酶链反应结果表明，运行 150 天后，在高盐度和低盐度生物反应器中，AOA 比 AOB 丰度分别高 1.6-54 倍和 2.3-81.1 倍。7种化学物质对富AOA污泥的抑制试验结果表明，0.5mg/L的苯酚、苯、甲苯和吡啶对富AOA污泥的硝化抑制率超过55%。然而，与 AOB 相比，AOA 对二甲硫醚和烯丙基硫脲显示出更高的耐受性。乙二胺对 AOA 和 AOB 具有相似的抑制作用。