Saccharum Granorum (YiTang) is a nutrient-rich food which could significantly treat chronic atrophic gastritis (CAG). However, its mechanism remains unclear. In the present work, a mitochondria-specific metabolomics approach based on ultra high performance liquid chromatography coupled with quadrupole - Exactive mass spectrometry (UHPLC-Q exactive MS) was carried out to evaluate its protection against energy dysfunction induced by CAG. Sixteen endogenous metabolites were firstly screened out to characterize the mitochondria dysfunction associated with CAG. Pretreatment of YiTang could significantly regulate these targeted metabolites involved into fatty acid β-oxidation pathway and amino acid metabolism. Network pharmacology further showed the upstream proteins related to these targeted metabolites exerted good affinities with ingredients of YiTang. These findings provided a unique perspective on localized metabolic regulation of YiTang in rats with CAG, which gave us new insights into the pathogenesis of CAG, and its targets for clinical diagnosis and treatment.

糖精（一糖）是一种营养丰富的食品，可以显着治疗慢性萎缩性胃炎（CAG）。但是，其机制仍不清楚。在目前的工作中，基于超高效液相色谱结合四极杆-主动质谱（UHPLC-Q精确质谱）的线粒体特异性代谢组学方法进行了评估，以评估其对CAG诱导的能量功能障碍的保护作用。首先筛选出16种内源性代谢产物，以表征与CAG相关的线粒体功能障碍。益糖预处理可显着调节这些与脂肪酸β有关的靶向代谢物-氧化途径和氨基酸代谢。网络药理学进一步表明，与这些靶向代谢物相关的上游蛋白与益糖的成分具有良好的亲和力。这些发现为益糖对大鼠CAG的局部代谢调节提供了独特的见解，这为我们提供了对CAG的发病机理及其临床诊断和治疗目标的新见解。