Cell migration plays fundamental roles in the development and maintenance of organisms such as wound healing and embryonic development, and cell migration is affected by the surrounding nanostructure of the extracellular matrix. Many researchers have developed various nanotopographical structures to investigate cell migration behaviors in response to the nanostructures, however, it is hard to draw firm conclusions about a relationship between surface topography and cell migration due to the contradictory results. Here, as one of the possible approaches to investigate the cell migration behaviors on the nanotopographies, we developed isotropic nanopore surfaces with high and low aspect ratios to have decreased and increased adherable areas, respectively. The nanopore surface with high aspect ratio had decreased adherable area of 86 % by providing the cells to adhere only on the top surface of the nanopores, while the nanopore surface with low aspect ratio had increased adherable area of 130 % by providing the cells to perceive both on the top surface and the bottom surface of the nanopores. In contrast to our expectation, the migration speeds of fibroblast cells were both promoted on the nanopore surfaces compared to the flat surface, regardless of the increase or decrease in adherable area. We found that the maturation of the focal adhesions (FAs) at the peripheral region is a key factor determining the adhesion strength of the fibroblast cells, which in turn affects cell migration. Our findings may widen our understanding of the cell migration behaviors on the nanostructured surface according to the formation of the FAs.

细胞迁移在伤口愈合和胚胎发育等生物体的发育和维持中起着重要作用，细胞迁移受周围细胞外基质纳米结构的影响。许多研究人员已经开发了各种纳米拓扑结构来研究响应纳米结构的细胞迁移行为，然而，由于相互矛盾的结果，很难得出关于表面拓扑和细胞迁移之间关系的确切结论。在这里，作为研究纳米形貌上细胞迁移行为的可能方法之一，我们开发了具有高和低纵横比的各向同性纳米孔表面，分别减少和增加了可粘附区域。高纵横比的纳米孔表面通过使细胞仅粘附在纳米孔的顶面上使粘附面积减少了 86 %，而低纵横比的纳米孔表面通过提供细胞感知而使粘附面积增加了 130 %。在纳米孔的顶面和底面。与我们的预期相反，与平坦表面相比，成纤维细胞在纳米孔表面上的迁移速度都得到了提高，而不管可粘附面积的增加或减少。我们发现外周区域粘着斑（FAs）的成熟是决定成纤维细胞粘附强度的关键因素，进而影响细胞迁移。