This study investigated the systematic identification and characterization of metabolic changes in wild and SCC of H. pinifolia using GC–MS based metabolomics approach. Finally, we identified 98 metabolites in wild and 125 metabolites in SCC and determined their relative abundance. Identified metabolites were mapped to general biochemical pathways and these metabolites spanned 77 primary and secondary metabolism pathways in wild, while 73 metabolism pathways in SCC. Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of integration data demonstrated that wild and SCC showed profound biochemical changes including glutathione metabolism, glutamate metabolism, phenylalanine and tyrosine metabolism in wild, while SCC caused signification variations in citric acid metabolism, glycolysis, gluconeogenesis, oxidative phosphorylation related pathways respectively. Random forest analysis showed top 15 metabolites and their correct classification. Present findings provide baseline information on H. pinifolia metabolism in the marine and artificial environments. Moreover, metabolomics data obtained from H. pinifolia may be used to compare the metabolites associated with stress response which would facilitate further dissection mechanism of stress tolerance of other seagrass species in the marine environments.

这项研究调查了系统的鉴定和表征的野生和松果的鳞状细胞癌的代谢变化使用基于GC-MS的代谢组学方法。最后，我们在野生动植物中鉴定了98种代谢物，在SCC中鉴定了125种代谢物，并确定了它们的相对丰度。鉴定出的代谢物被定位到一般的生化途径，这些代谢物在野生环境中跨越了77个主要和次要代谢途径，而在SCC中则跨越了73个代谢途径。《京都市基因与基因组百科全书》（KEGG）整合数据的途径分析表明，野生和SCC在野外显示出深刻的生化变化，包括谷胱甘肽代谢，谷氨酸代谢，苯丙氨酸和酪氨酸代谢，而SCC引起柠檬酸代谢，糖酵解，糖异生的意义变化。分别与氧化磷酸化有关的途径。随机森林分析显示了前15种代谢物及其正确分类。在海洋和人工环境中，H。pinifolia代谢。此外，从松叶螺旋体获得的代谢组学数据可用于比较与应激反应相关的代谢产物，这将有助于进一步剖析海洋环境中其他海草物种的胁迫耐受性的机制。