Abstract Microalgae feed production is a major cost in bivalve aquaculture. Its efficiency is increased by scaling it up under automated control of environmental conditions. The initial cost of commercially available systems can be prohibitive. Therefore, a large volume full-scale photobioreactor built of low-cost, readily available materials was developed and tested. A cone-bottom, polyethylene tank was internally illuminated with submersible fluorescent lamps and equipped with a monitoring and control system that measured temperature, pH and optical density of the microalgal culture. Injection of CO2 was automated to maintain pH within a target range. System performance was evaluated by growing four batch cultures of Tetraselmis chuii. Temperature inside the photobioreactor was 29.5 ± 2.38 °C (mean ± SD, range 21−35 °C) and pH was 7.56 ± 0.87 (mean ± SD, range 5.29–8.97). Optimal harvest time was after 300 h (12.5 days) of growth, yielding 1700 L of microalgae at a density of 2500 cells/ μl (1200 cells/ μl/ m2 illuminated surface area). With 24 h illumination, the fluorescent lights, air pump and monitoring/ control device consumed 14.9, 1.9 and 0.1kwh/ day, respectively. Assuming the microalgae are harvested at their optimal density after 12.5 days, each batch would consume 211kwh or 0.124kwh/ liter. The photobioreactor described provides an economical option for growing large amounts of microalgae for aquaculture feed and other purposes.

中文翻译：

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用于微藻生产的低成本、自动化、大规模光生物反应器的设计和性能

摘要 微藻饲料生产是双壳贝类养殖的主要成本。它的效率通过在环境条件的自动控制下扩大规模来提高。商用系统的初始成本可能高得令人望而却步。因此，开发并测试了一种由低成本、现成的材料制成的大容量全尺寸光生物反应器。锥底聚乙烯罐内部用潜水荧光灯照明，并配备监测和控制系统，可测量微藻培养物的温度、pH 值和光密度。自动注入 CO2 以将 pH 值保持在目标范围内。通过培养四批 Tetraselmis chuii 来评估系统性能。光生物反应器内的温度为 29.5 ± 2.38 °C（平均值 ± SD，范围 21-35 °C），pH 值为 7.56 ± 0。87（平均值±SD，范围5.29-8.97）。最佳收获时间是在生长 300 小时（12.5 天）后，以 2500 个细胞/μl（1200 个细胞/μl/m2 光照表面积）的密度产生 1700 L 微藻。在 24 小时照明下，荧光灯、气泵和监控/控制装置分别消耗 14.9、1.9 和 0.1 千瓦时/天。假设微藻在 12.5 天后以其最佳密度收获，每批将消耗 211 千瓦时或 0.124 千瓦时/升。所描述的光生物反应器为养殖大量用于水产养殖饲料和其他目的的微藻提供了一种经济的选择。气泵和监控/控制装置分别消耗14.9、1.9和0.1kwh/天。假设微藻在 12.5 天后以其最佳密度收获，每批将消耗 211 千瓦时或 0.124 千瓦时/升。所描述的光生物反应器为养殖大量用于水产养殖饲料和其他目的的微藻提供了一种经济的选择。气泵和监控/控制装置分别消耗14.9、1.9和0.1kwh/天。假设微藻在 12.5 天后以其最佳密度收获，每批将消耗 211 千瓦时或 0.124 千瓦时/升。所描述的光生物反应器为养殖大量用于水产养殖饲料和其他目的的微藻提供了一种经济的选择。