FRET-based colorimetric and ratiometric sensor for visualizing pH change and application for bioimaging in living cells, bacteria and zebrafish,Analytica Chimica Acta

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FRET-based colorimetric and ratiometric sensor for visualizing pH change and application for bioimaging in living cells, bacteria and zebrafish
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.aca.2020.06.031
Xiaojun He ₁ , Feng Ding ₂ , Wei Xu ₁ , Chuchu Xu ₁ , Yahui Li ₁ , Yuna Qian ₃ , Shengzhe Zhao ₃ , Hong Chen ₄ , Jianliang Shen ₅

Affiliation

Acid-alkaline balance plays a crucial role in all biological processes. Accordingly, monitoring pH changes will help us to understand the functional status of these physiological and pathological processes. Though fluorescent probes may be a useful tool for detecting pH changes, and there are many limitations to currently available probes, such as background interference, potential cytotoxicity, and poor cell permeability, which call for a solution urgently. In this work, a rhodamine-derived colorimetric and ratiometric sensor (Rh-HN) was fabricated for monitoring pH change via the mechanism of fluorescence resonance energy transfer (FRET). Rh-HN has been shown to possess several advantages over other probes, such as high sensitivity, outstanding permeability, and low toxicity. Besides, the fluorescence intensity ratio (F526/F592) of Rh-HN displays a pH-sensitive response from 2.0 to 7.5 (pKa = 5.05) and linear response from pH 3.8 to 6.4, which was desirable for mapping pH change in the biological systems. Besides, the results indicated that Rh-HN generated a pH-dependent response regulated by switchable forms between closed and opened spirolactam ring. Overall, Rh-HN has accomplished sensing and mapping of pH in living cells, bacteria, and zebrafish. Those results demonstrated that the great potential of Rh-HN in sensing and visualizing pH in the living biosystem.

中文翻译：

基于 FRET 的比色和比率传感器，用于可视化 pH 值变化以及在活细胞、细菌和斑马鱼中的生物成像应用

酸碱平衡在所有生物过程中起着至关重要的作用。因此，监测 pH 值变化将有助于我们了解这些生理和病理过程的功能状态。尽管荧光探针可能是检测 pH 变化的有用工具，但目前可用的探针存在许多局限性，例如背景干扰、潜在的细胞毒性和细胞通透性差，迫切需要解决方案。在这项工作中，制造了一种罗丹明衍生的比色和比率传感器（Rh-HN），用于通过荧光共振能量转移（FRET）机制监测 pH 值变化。Rh-HN 已被证明具有优于其他探针的几个优点，例如高灵敏度、出色的渗透性和低毒性。除了，Rh-HN 的荧光强度比 (F526/F592) 显示出 2.0 到 7.5 (pKa = 5.05) 的 pH 敏感响应和从 pH 3.8 到 6.4 的线性响应，这对于绘制生物系统中的 pH 变化是理想的。此外，结果表明，Rh-HN 产生了一种 pH 依赖性反应，该反应由封闭和开放的螺内酰胺环之间的可转换形式调节。总体而言，Rh-HN 已经完成了活细胞、细菌和斑马鱼中 pH 值的传感和绘图。这些结果表明，Rh-HN 在传感和可视化活生物系统中的 pH 值方面具有巨大潜力。结果表明，Rh-HN 产生了一种 pH 依赖性反应，该反应由封闭和开放的螺内酰胺环之间的可转换形式调节。总体而言，Rh-HN 已经完成了活细胞、细菌和斑马鱼中 pH 值的传感和绘图。这些结果表明，Rh-HN 在传感和可视化活生物系统中的 pH 值方面具有巨大潜力。结果表明，Rh-HN 产生了一种 pH 依赖性反应，该反应由封闭和开放的螺内酰胺环之间的可转换形式调节。总体而言，Rh-HN 已经完成了活细胞、细菌和斑马鱼中 pH 值的传感和绘图。这些结果表明，Rh-HN 在传感和可视化活生物系统中的 pH 值方面具有巨大潜力。

更新日期：2020-08-01

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