Abstract—

The impact of two strains of Pseudomonas bacteria and natural zeolite on the growth and elemental composition of barley plants was studied in an agrogray soil (Luvisol) artificially contaminated with cadmium in pot experiments. Application of P. fluorescens 21, or P. putida 23, or zeolite eliminated the heavy metal toxicity for plants. The cumulative effect of co-application of P. fluorescens 21 and zeolite was insignificant. The bacteria- or zeolite-mediated plant tolerance to cadmium was attributed to the enhanced root system development, decreased cadmium translocation into the roots, and improved mineral nutrition of the plants. Elevated nutrient uptake by the plants under the influence of bacteria and zeolite was the result of plant growth stimulation without significant changes in the concentrations of macronutrients N, P, K, Ca, and Mg, as well as Fe and micronutrients Zn, Mn, and Cu in plant tissues, including grain. Application of P. fluorescens 21 enhanced the Cd fixation in the soil organic matter in the first half of the growing season, which could be due to the metal sequestration by bacterial siderophores. Thus, application of the bacteria and natural zeolite can be recommended in the strategies for Cd-polluted soil remediation based on environment-friendly technologies.

中文翻译：

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利用植物促生根瘤菌和天然沸石修复镉污染的土壤

摘要-

在盆栽实验中，在人工污染了镉的农业土壤（Luvisol）中研究了两种假单胞菌细菌和天然沸石对大麦植物生长和元素组成的影响。施用荧光假单胞菌21或恶臭假单胞菌23或沸石消除了对植物的重金属毒性。共同应用荧光假单胞菌的累积效应21和沸石是微不足道的。细菌或沸石介导的植物对镉的耐受性归因于增强的根系发育，减少的镉转运到根中以及改善了植物的矿质营养。在细菌和沸石的影响下，植物对养分的吸收增加是植物生长刺激的结果，而常量养分N，P，K，Ca和Mg以及Fe和微量养分Zn，Mn和M的浓度没有明显变化。植物组织（包括谷物）中的Cu。荧光假单胞菌的应用21在生长季节的上半年增强了Cd对土壤有机质的固定，这可能是由于细菌铁载体螯合了金属所致。因此，可以在基于环境友好技术的Cd污染土壤修复策略中推荐使用细菌和天然沸石。