Purpose

Crosslinked poly(vinyl alcohol) (PVA) is a biomaterial that can be used for multiple cardiovascular applications. The success of implanted biomaterials is contingent on the properties of the material. A crucial consideration for blood-contacting devices is their potential to incite thrombus formation, which is dependent on the material surface properties. The goal of this study was to quantify the effect of different crosslinking methods of PVA hydrogels on in vitro thrombogenicity.

Methods

PVA was manufactured using three different crosslinking methods: 30% sodium trimetaphosphate (STMP), three 24 h freeze–thaw cycles (FT), and 2% glutaraldehyde-crosslinked (GA) to produce STMP-PVA, FT-PVA and GA-PVA, respectively. Expanded polytetrafluoroethylene (ePTFE) was used as a clinical control. As markers of thrombus formation, the degree of coagulation factor (F) XII activation, fibrin formation, and platelet adhesion were measured.

Results

The GA-PVA material increased FXII activation in the presence of cofactors compared to vehicle and increase platelet adhesion compared to other PVA surfaces. The STMP-PVA and FT-PVA materials had equivalent degrees of FXII activation, fibrin formation and platelet adhesion.

Conclusion

This work supports crosslinker dependent thrombogenicity of PVA hydrogels and advances our understanding of how the manufacturing of a PVA hydrogel affects its hemocompatibility.

中文翻译：

---

评估聚（乙烯醇）水凝胶的交联方法对血栓形成的影响。

目的

交联聚乙烯醇 (PVA) 是一种生物材料，可用于多种心血管应用。植入生物材料的成功取决于材料的特性。血液接触装置的一个关键考虑因素是它们可能引发血栓形成，这取决于材料的表面特性。本研究的目的是量化 PVA 水凝胶的不同交联方法对体外血栓形成的影响。

方法

PVA 使用三种不同的交联方法制造：30% 三偏磷酸钠 (STMP)、三个 24 小时冻融循环 (FT) 和 2% 戊二醛交联 (GA) 以生产 STMP-PVA、FT-PVA 和 GA-PVA ， 分别。膨体聚四氟乙烯 (ePTFE) 用作临床对照。作为血栓形成的标志物，测量了凝血因子 (F) XII 激活、纤维蛋白形成和血小板粘附的程度。

结果

与载体相比，GA-PVA 材料在辅因子存在的情况下增加了 FXII 活化，并与其他 PVA 表面相比增加了血小板粘附。STMP-PVA 和 FT-PVA 材料具有相同程度的 FXII 活化、纤维蛋白形成和血小板粘附。

结论

这项工作支持 PVA 水凝胶的交联剂依赖性血栓形成性，并促进了我们对 PVA 水凝胶的制造如何影响其血液相容性的理解。