BACKGROUND:The pathway of anthocyanin biosynthesis, and its alterations leading to berry colour modification, are well known in grape skin. This variability could affect both quantity and quality of pigment accumulation. OBJECTIVE:The present work is focused on 15 grapevine cultivars selected to represent a high variability in the phenotypical colour traits in order to highlight new polymorphisms related to the flavonoid pathway. METHODS:Twenty-one genes involved in the biosynthetic pathway of anthocyanins were studied via targeted resequencing and were correlated with phenotypic data (anthocyanin profiles and spectroscopy indices). RESULTS:Single nucleotide polymorphism (SNP) and InDel (insertion/deletion) polymorphisms were detected. Out of 1751 polymorphic loci, 68% were SNPs and 32% were InDels (568). Cluster analysis and SPLS-DA were used to investigate the genetic relationships among the cultivars, confirming the large range of phenotypical variability. Statistically significant correlations were detected between accumulation of 3’ anthocyanins and genetic polymorphisms in two structural genes and one transcription factor putatively involved in the anthocyanin biosynthetic pathway. CONCLUSIONS:The understanding of the polymorphisms related to the anthocyanin accumulation could support future selection of new pink table grape varieties with increased appeal on the consumers.

中文翻译：

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通过有针对性的重测序方法研究的葡萄品种花色苷积累中的功能障碍

背景：花色苷的生物合成途径及其导致浆果颜色修饰的改变在葡萄皮中是众所周知的。这种可变性会影响颜料堆积的数量和质量。目的：目前的工作集中在15个葡萄品种上，这些葡萄品种被选为表型颜色性状的高变异性，以突出与黄酮途径相关的新的多态性。方法：通过有针对性的重测序研究花色苷生物合成途径中的二十一个基因，并将其与表型数据（花色素苷谱和光谱指数）相关。结果：检测到单核苷酸多态性（SNP）和InDel（插入/缺失）多态性。在1751个多态位点中，SNP占68％，InDels占32％（568）。聚类分析和SPLS-DA用于调查品种之间的遗传关系，证实了大范围的表型变异性。在两个结构基因和一个假定参与花色苷生物合成途径的转录因子的3'花色苷积累和遗传多态性之间检测到统计学上显着的相关性。结论：对与花青素积累有关的多态性的理解可以为将来选择新的粉红色食用葡萄品种提供支持，从而吸引更多消费者。在两个结构基因和一个假定参与花色苷生物合成途径的转录因子的3'花色苷积累和遗传多态性之间检测到统计学上显着的相关性。结论：对与花青素积累有关的多态性的理解可以为将来选择新的粉红色食用葡萄品种提供支持，从而吸引更多消费者。在两个结构基因和一个假定参与花色苷生物合成途径的转录因子的3'花色苷积累和遗传多态性之间检测到统计学上显着的相关性。结论：对与花青素积累有关的多态性的理解可以为将来选择新的粉红色食用葡萄品种提供支持，从而吸引更多消费者。