The interaction of deoxyribonucleic acid (DNA) with polymeric nano- and microcarriers has attracted significant attention in the studies on DNA diagnostic assays, DNA isolation and particularly gene delivery and cancer therapy agents. Therefore, in this study, plain poly(glycerol dimethacrylate) (poly(GDMA)) and poly(dimethylaminoethyl methacrylate)-grafted poly(GDMA) microgel particles were synthesized for high single-stranded DNA (ssDNA) adsorption and desorption capacity. Temperature- and pH-sensitive uniform poly(GDMA) particles were synthesized through a two-stage procedure. A GDMA dispersion polymerization procedure was used in the first stage of the study to obtain microgel particles with approximately 900 nm size. The surface-initiated atom transfer radical polymerization procedure, a living-polymerization technique of 2-dimethylaminoethyl methacrylate (DMAEM), was used in the second stage of the study to obtain polycationic molecular brushes on microgel particles. The resulting temperature- and pH-responsive microgels were used as a sorbent for ssDNA adsorption. Compared with commonly used sorbents, reasonably high ssDNA adsorptions were achieved with the proposed sorbent. The adsorbed ssDNA was desorbed from the sorbent at a high yield. The adsorption and desorption behaviors make poly(DMAEM)-grafted poly(GDMA) microgel particles a promising carrier/sorbent for ssDNA.

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用于诊断分析和基因传递系统的聚合物微载体

脱氧核糖核酸（DNA）与聚合的纳米和微载体的相互作用在DNA诊断测定，DNA分离，尤其是基因传递和癌症治疗剂的研究中引起了极大的关注。因此，在这项研究中，合成了普通的聚二甲基丙烯酸甘油酯（GDMA）和聚甲基丙烯酸二甲基氨基乙基酯接枝的聚GDMA微凝胶颗粒，以实现高的单链DNA（ssDNA）吸附和解吸能力。通过两步法合成了对温度和pH敏感的均质聚（GDMA）颗粒。在研究的第一阶段使用了GDMA分散聚合程序，以获得约900 nm尺寸的微凝胶颗粒。表面引发的原子转移自由基聚合过程，在研究的第二阶段，使用了甲基丙烯酸2-二甲基氨基乙基酯（DMAEM）的活性聚合技术在微凝胶颗粒上获得聚阳离子分子刷。所得的温度和pH响应微凝胶用作ssDNA吸附的吸附剂。与常用的吸附剂相比，所提出的吸附剂可实现相当高的ssDNA吸附。吸附的ssDNA以高收率从吸附剂上解吸。吸附和解吸行为使聚（DMAEM）接枝的聚（GDMA）微凝胶颗粒成为ssDNA的有希望的载体/吸附剂。与常用的吸附剂相比，所提出的吸附剂可实现相当高的ssDNA吸附。吸附的ssDNA以高收率从吸附剂上解吸。吸附和解吸行为使聚（DMAEM）接枝的聚（GDMA）微凝胶颗粒成为ssDNA的有希望的载体/吸附剂。与常用的吸附剂相比，所提出的吸附剂可实现相当高的ssDNA吸附。吸附的ssDNA以高收率从吸附剂上解吸。吸附和解吸行为使聚（DMAEM）接枝的聚（GDMA）微凝胶颗粒成为ssDNA的有希望的载体/吸附剂。