In this paper, preparation and characterization of a nanofibrous scaffold comprising both chitosan (CS) and gum tragacanth (GT) are reported for the first time. CS and GT were separately blended with poly(vinyl alcohol) (PVA) and simultaneously electrospun by a double-nozzle electrospinning apparatus, and the resulting nanofibrous mats were compared with CS/PVA and GT/PVA electrospun ones. Scanning electron microscopy images showed uniform bead-free nanofibers with some merging areas and an average fiber diameter of ∼273 nm for CS-PVA/GT-PVA. The ultimate tensile strength and strain at break of the hybrid nanofibers were ∼20 MPa and ∼9%, respectively, which were significantly higher than those of the CS/PVA and GT/PVA nanofibers. The CS-PVA/GT-PVA nanofibrous mats also showed a water droplet contact angle value (∼31°) between those of the CS/PVA and GT/PVA nanofibrous mats. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay using L929 fibroblast cells indicated more biocompatibility of the CS-PVA/GT-PVA hybrid nanofibers compared with the CS/PVA and GT/PVA ones. The obtained results showed that the CS-PVA/GT-PVA hybrid nanofibrous scaffold might be useful for tissue engineering applications.

中文翻译：

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壳聚糖/黄tr胶/ PVA杂合纳米纤维支架，用于组织工程应用

在本文中，首次报道了同时包含壳聚糖（CS）和黄aga胶（GT）的纳米纤维支架的制备和表征。将CS和GT分别与聚乙烯醇（PVA）混合，并同时通过双喷嘴静电纺丝设备进行电纺丝，然后将所得的纳米纤维垫与CS / PVA和GT / PVA电纺丝进行比较。扫描电子显微镜图像显示，CS-PVA / GT-PVA的均匀无珠纳米纤维具有一些融合区域，平均纤维直径约为273 nm。杂化纳米纤维的极限拉伸强度和断裂应变分别为〜20 MPa和〜9％，显着高于CS / PVA和GT / PVA纳米纤维。CS-PVA / GT-PVA纳米纤维垫还显示出CS / PVA和GT / PVA纳米纤维垫之间的水滴接触角值（约31°）。使用L929成纤维细胞的MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑）测定表明，CS-PVA / GT-PVA杂合纳米纤维的生物相容性比CS / PVA和GT / PVA的。获得的结果表明，CS-PVA / GT-PVA杂化纳米纤维支架可能对组织工程应用有用。