当前位置：
X-MOL 学术
›
Int. J. Nanomed.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles.
International Journal of Nanomedicine ( IF 6.6 ) Pub Date : 2020-06-03 , DOI: 10.2147/ijn.s244686 Diya Leng 1, 2 , Yan Li 3 , Jie Zhu 4 , Ruizhen Liang 1, 5 , Cuifeng Zhang 1, 2 , Yang Zhou 1, 2 , Mingming Li 1, 2 , Ying Wang 1, 2 , Di Rong 1, 2 , Daming Wu 1, 2 , Jin Li 1, 6
International Journal of Nanomedicine ( IF 6.6 ) Pub Date : 2020-06-03 , DOI: 10.2147/ijn.s244686 Diya Leng 1, 2 , Yan Li 3 , Jie Zhu 4 , Ruizhen Liang 1, 5 , Cuifeng Zhang 1, 2 , Yang Zhou 1, 2 , Mingming Li 1, 2 , Ying Wang 1, 2 , Di Rong 1, 2 , Daming Wu 1, 2 , Jin Li 1, 6
Affiliation
Background: Mesoporous calcium-silicate nanoparticles (MCSNs) have good prospects in the medical field due to their great physicochemical characteristics, antibacterial activity and drug delivery capacity. This study was to analyze the antibiofilm activity and mechanisms of silver (Ag) and zinc (Zn) incorporated MCSNs (Ag/Zn-MCSNs) with different percentages of Ag and Zn.
Methods: Ag/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by Enterococcus faecalis (E. faecalis) treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by E. faecalis biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with E. faecalis. Then, SEM and CLSM were used to observe the survival of E. faecalis on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits.
Results: The Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against E. faecalis biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation.
Conclusion: Ag/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.
中文翻译：
纳米银和纳米锌结合的介孔硅酸钙纳米粒子的抗菌膜活性和机制。
背景：介孔硅酸钙纳米粒子（MCSNs）具有良好的理化特性、抗菌活性和药物输送能力，在医学领域具有良好的应用前景。本研究旨在分析银 (Ag) 和锌 (Zn) 结合的 MCSNs (Ag/Zn-MCSNs) 与不同比例的 Ag 和 Zn 的抗菌膜活性和机制。
方法：制备并表征了Ag/Zn(1:9,摩尔比)-MCSNs和Ag/Zn(9:1,摩尔比)-MCSNs。使用 TEM 观察了用 Ag/Zn-MCSNs 处理的粪肠球菌( E. faecalis )对纳米颗粒的内吞作用，以探索其抗菌机制。粪肠球菌检测不同Ag和Zn配比的Ag/Zn-MCSNs的抗菌膜活性人体根部的生物膜模型。用不同的Ag/Zn-MCSNs预处理的人根与粪肠球菌一起培养。然后用SEM和CLSM观察根管壁上粪肠球菌的存活情况。通过 CCK8 试剂盒测试纳米颗粒的细胞毒性。
结果： Ag/Zn-MCSNs释放Ag +并破坏细胞膜以杀死细菌。含有 Ag 的 MCSNs 对粪肠球菌具有抗菌活性不同程度的生物膜，它们可以粘附在牙本质表面，从而获得持续的抗菌作用。然而，MTA、MCSNs和Zn-MCSNs不能明显破坏细菌生物膜。MCSNs、Ag/Zn(1:1,摩尔比)-MCSNs和Ag/Zn(1:9)-MCSNs没有表现出明显的细胞毒性，而Ag-MCSNs和Ag/Zn(9:1)-MCSNs表现出细胞毒性。Zn-MCSNs 可以轻微促进细胞增殖。
结论： Ag/Zn-MCSNs具有良好的抗生物膜活性。通过调整Ag和Zn的比例，它们可以在抗菌活性和细胞毒性之间取得适当的平衡。Ag/Zn-MCSNs有望成为一种新型的根管消毒剂或根管治疗封闭剂。
更新日期：2020-06-03
Methods: Ag/Zn(1:9, molar ratio)-MCSNs and Ag/Zn(9:1, molar ratio)-MCSNs were prepared and characterized. Endocytosis of nanoparticles by Enterococcus faecalis (E. faecalis) treated with Ag/Zn-MCSNs was observed using TEM to explore the antibacterial mechanisms. The antibiofilm activity of Ag/Zn-MCSNs with different ratios of Ag and Zn was tested by E. faecalis biofilm model in human roots. The human roots pretreated by different Ag/Zn-MCSNs were cultured with E. faecalis. Then, SEM and CLSM were used to observe the survival of E. faecalis on the root canal wall. Cytotoxicity of the nanoparticles was tested by CCK8 kits.
Results: The Ag/Zn-MCSNs release Ag+ and destroy the cell membranes to kill bacteria. The MCSNs containing Ag showed antibacterial activity against E. faecalis biofilms in different degrees, and they can adhere to dentin surfaces to get a continuous antibacterial effect. However, MTA, MCSNs and Zn-MCSNs could not disrupt the bacterial biofilms obviously. MCSNs, Ag/Zn(1:1, molar ratio)-MCSNs and Ag/Zn(1:9)-MCSNs showed no obvious cytotoxicity, while Ag-MCSNs and Ag/Zn(9:1)-MCSNs showed cytotoxicity. Zn-MCSNs can slightly promote cell proliferation.
Conclusion: Ag/Zn-MCSNs have good antibiofilm activity. They might achieve an appropriate balance between the antibacterial activity and cytotoxicity by adjusting the ratio of Ag and Zn. Ag/Zn-MCSNs are expected to be a new type of root canal disinfectant or sealer for root canal treatment.
中文翻译：
纳米银和纳米锌结合的介孔硅酸钙纳米粒子的抗菌膜活性和机制。
背景：介孔硅酸钙纳米粒子（MCSNs）具有良好的理化特性、抗菌活性和药物输送能力，在医学领域具有良好的应用前景。本研究旨在分析银 (Ag) 和锌 (Zn) 结合的 MCSNs (Ag/Zn-MCSNs) 与不同比例的 Ag 和 Zn 的抗菌膜活性和机制。
方法：制备并表征了Ag/Zn(1:9,摩尔比)-MCSNs和Ag/Zn(9:1,摩尔比)-MCSNs。使用 TEM 观察了用 Ag/Zn-MCSNs 处理的粪肠球菌( E. faecalis )对纳米颗粒的内吞作用，以探索其抗菌机制。粪肠球菌检测不同Ag和Zn配比的Ag/Zn-MCSNs的抗菌膜活性人体根部的生物膜模型。用不同的Ag/Zn-MCSNs预处理的人根与粪肠球菌一起培养。然后用SEM和CLSM观察根管壁上粪肠球菌的存活情况。通过 CCK8 试剂盒测试纳米颗粒的细胞毒性。
结果： Ag/Zn-MCSNs释放Ag +并破坏细胞膜以杀死细菌。含有 Ag 的 MCSNs 对粪肠球菌具有抗菌活性不同程度的生物膜，它们可以粘附在牙本质表面，从而获得持续的抗菌作用。然而，MTA、MCSNs和Zn-MCSNs不能明显破坏细菌生物膜。MCSNs、Ag/Zn(1:1,摩尔比)-MCSNs和Ag/Zn(1:9)-MCSNs没有表现出明显的细胞毒性，而Ag-MCSNs和Ag/Zn(9:1)-MCSNs表现出细胞毒性。Zn-MCSNs 可以轻微促进细胞增殖。
结论： Ag/Zn-MCSNs具有良好的抗生物膜活性。通过调整Ag和Zn的比例，它们可以在抗菌活性和细胞毒性之间取得适当的平衡。Ag/Zn-MCSNs有望成为一种新型的根管消毒剂或根管治疗封闭剂。
京公网安备 11010802027423号