NBS-LRR (nucleotide-binding site and leucine-rich repeat) is one of the largest resistance gene families in plants. The completion of the genome sequencing of wild tomato Solanum pimpinellifolium provided an opportunity to conduct a comprehensive analysis of the NBS-LRR gene superfamily at the genome-wide level. In this study, gene identification, chromosome mapping, and phylogenetic analysis of the NBS-LRR gene family were analyzed using the bioinformatics methods. The results revealed 245 NBS-LRRs in total, similar to that in the cultivated tomato. These genes are unevenly distributed on 12 chromosomes, and ~59.6% of them form gene clusters, most of which are tandem duplications. Phylogenetic analysis divided the NBS-LRRs into 2 subfamilies (CNL-coiled-coil NBS-LRR and TNL-TIR NBS-LRR), and the expansion of the CNL subfamily was more extensive than the TNL subfamily. Novel conserved structures were identified through conserved motif analysis between the CNL and TNL subfamilies. Compared with the NBS-LRR sequences from the model plant Arabidopsis thaliana, wide genetic variation occurred after the divergence of S. pimpinellifolium and A thaliana. Species-specific expansion was also found in the CNL subfamily in S. pimpinellifolium. The results of this study provide the basis for the deeper analysis of NBS-LRR resistance genes and contribute to mapping and isolation of candidate resistance genes in S. pimpinellifolium.

NBS-LRR（核苷酸结合位点和富含亮氨酸的重复序列）是植物中最大的抗性基因家族之一。野生番茄茄皮植物基因组测序的完成提供了在全基因组水平上对NBS-LRR基因超家族进行全面分析的机会。在这项研究中，使用生物信息学方法分析了NBS-LRR基因家族的基因鉴定，染色体作图和系统发育分析。结果显示总共有245个NBS-LRR，与栽培番茄相似。这些基因在12条染色体上分布不均，其中约59.6％形成基因簇，其中大多数是串联重复。系统发育分析将NBS-LRRs分为2个亚科（CNL盘绕NBS-LRR和TNL-TIR NBS-LRR），CNL的扩展比TNL的扩展。通过CNL和TNL亚家族之间的保守基序分析，鉴定出了新颖的保守结构。与模型工厂的NBS-LRR序列比较拟南芥（Arabidopsis thaliana），宽皮链球菌（S. pimpinellifolium）与拟南芥（A thaliana）发生分化后发生了广泛的遗传变异。在小叶葡萄球菌的CNL亚科中也发现了物种特异性的扩增。本研究结果为进一步分析NBS-LRR抗性基因奠定了基础，并有助于对S. pimpinellifolium中的候选抗性基因进行定位和分离。