We have developed stable chitosan colloids over a wide pH range without cross-linkers or gelling agents. The colloid was prepared using chitosan nanoparticle obtained from pulverization of bulk chitosan powder, followed by surface treatment using small amount of ascorbic acid (AA) and polyglycerol monostearate (PGMS) in water. Chitosan nanoparticles were well dispersed in a diluted AA solution due to the protonation of the chitosan chain on the surface. And then, the addition of PGMS led them to exhibit highly stable dispersion even in alkali conditions and 50 °C. The hydrodynamic diameter of the colloid was monitored using dynamic light scattering and the real image of the colloid was obtained using cryo-electron microscope measurement. This chitosan colloid will be useful for developing food ingredients or drug carrier templates that should be stable over a wide pH range.

中文翻译：

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通过吸附聚甘油单硬脂酸酯分散在宽 pH 范围内稳定的壳聚糖纳米颗粒

我们已经开发出在很宽的 pH 范围内稳定的壳聚糖胶体，无需交联剂或胶凝剂。使用从散装壳聚糖粉末粉碎获得的壳聚糖纳米颗粒制备胶体，然后在水中使用少量抗坏血酸 (AA) 和聚甘油单硬脂酸酯 (PGMS) 进行表面处理。由于壳聚糖链在表面的质子化，壳聚糖纳米颗粒很好地分散在稀释的 AA 溶液中。然后，PGMS 的加入使它们即使在碱性条件和 50 °C 下也表现出高度稳定的分散性。使用动态光散射监测胶体的流体动力学直径，并使用低温电子显微镜测量获得胶体的真实图像。