Nanoscale laser-induced breakdown spectroscopy imaging reveals chemical distribution with subcellular resolution,Nanoscale Advances

当前位置： X-MOL 学术 › Nanoscale Adv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Nanoscale laser-induced breakdown spectroscopy imaging reveals chemical distribution with subcellular resolution
Nanoscale Advances ( IF 4.6 ) Pub Date : 2020-06-29 , DOI: 10.1039/d0na00380h
Yifan Meng ₁ , Chaohong Gao ₁ , Zheng Lin ₁ , Wei Hang _{1,

2} , Benli Huang ₁

Affiliation

Understanding chemical compositions is one of the most important parts in exploring the microscopic world. As a simple method for elemental detection, laser-induced breakdown spectroscopy (LIBS) is widely used in materials, geological and life science fields. However, due to the long-existing limitation in spatial resolution, it is difficult for LIBS to play an analytical role in the field of micro-world. Herein, we first report a reliable nanoscale resolution LIBS imaging technique by introducing a sampling laser with a micro-lensed fiber. Through the emission enhancement using the double-pulse laser, we obtained the spectral signal from a sampling crater of less than 500 nanometers in diameter, and visualized the chemical distribution of the self-made grid sample, SIM chip and nano-particles in single cells. The relative limits of detection (RLODs) of In and absolute limits of detection (ALODs) of Al can reach 0.6% and 18.3 fg, respectively.

中文翻译：

纳米级激光诱导击穿光谱成像揭示了具有亚细胞分辨率的化学分布

了解化学成分是探索微观世界最重要的部分之一。激光诱导击穿光谱（LIBS）作为一种简单的元素检测方法，广泛应用于材料、地质和生命科学领域。然而，由于空间分辨率长期存在的局限性，LIBS很难在微观世界领域发挥分析作用。在这里，我们首先通过引入带有微透镜光纤的采样激光器报告了一种可靠的纳米级分辨率 LIBS 成像技术。通过双脉冲激光的发射增强，我们获得了直径小于500纳米的采样坑的光谱信号，并可视化了自制的网格样品、SIM芯片和纳米粒子在单细胞中的化学分布.

更新日期：2020-06-29

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文