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Supramolecular assembly of DNA-constructed vesicles.
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-29 , DOI: 10.1039/d0nr04103c Simon Rothenbühler 1 , Ioan Iacovache 2 , Simon M Langenegger 1 , Benoît Zuber 2 , Robert Häner 1
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-29 , DOI: 10.1039/d0nr04103c Simon Rothenbühler 1 , Ioan Iacovache 2 , Simon M Langenegger 1 , Benoît Zuber 2 , Robert Häner 1
Affiliation
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The self-assembly of DNA hybrids possessing tetraphenylethylene sticky ends at both sides into vesicular architectures in aqueous medium is demonstrated. Cryo-electron microscopy reveals the formation of different types of morphologies from the amphiphilic DNA-hybrids. Depending on the conditions, either an extended (sheet-like) or a compact (columnar) alignment of the DNA hybrids is observed. The different modes of DNA arrangement lead to the formation of vesicles appearing either as prolate ellipsoids (type I) or as spheres (type II). The type of packing has a significant effect on the accessibility of the DNA, as evidenced by intercalation and light-harvesting experiments. Only the vesicles exhibiting the sheet-like DNA alignment are accessible for intercalation by ethidium bromide or for the integration of chromophore-labelled DNA via a strand exchange process. The dynamic nature of type I vesicles enables their elaboration into artificial light-harvesting complexes by DNA-guided introduction of Cy3-acceptor chromophores. DNA-constructed vesicles of the kind shown here represent versatile intermediates that are amenable to further modification for tailored nanotechnology applications.
中文翻译：
DNA构成的囊泡的超分子组装。
证明了在水性介质中，两侧均具有四苯基乙烯粘性末端的DNA杂种的自组装成为水泡结构。低温电子显微镜揭示了由两亲性DNA杂交体形成的不同类型的形态。根据条件，可以观察到DNA杂合体的延长的（片状）或紧凑的（柱状）比对。DNA排列的不同模式导致形成长椭圆形（I型）或球体（II型）的囊泡形成。包装的类型对DNA的可及性有重要影响，插入和光收集实验证明了这一点。只有表现出片状DNA排列的囊泡才能被溴化乙锭插入或整合发色团标记的DNA通过链交换过程。I型囊泡的动态性质使它们能够通过DNA引导的Cy3受体发色团的合成而精细化为人造光捕获复合物。此处显示的那种由DNA构成的囊泡代表了通用的中间体，它们可以针对定制的纳米技术应用进行进一步修饰。
更新日期：2020-07-02
中文翻译：
DNA构成的囊泡的超分子组装。
证明了在水性介质中，两侧均具有四苯基乙烯粘性末端的DNA杂种的自组装成为水泡结构。低温电子显微镜揭示了由两亲性DNA杂交体形成的不同类型的形态。根据条件，可以观察到DNA杂合体的延长的（片状）或紧凑的（柱状）比对。DNA排列的不同模式导致形成长椭圆形（I型）或球体（II型）的囊泡形成。包装的类型对DNA的可及性有重要影响，插入和光收集实验证明了这一点。只有表现出片状DNA排列的囊泡才能被溴化乙锭插入或整合发色团标记的DNA通过链交换过程。I型囊泡的动态性质使它们能够通过DNA引导的Cy3受体发色团的合成而精细化为人造光捕获复合物。此处显示的那种由DNA构成的囊泡代表了通用的中间体，它们可以针对定制的纳米技术应用进行进一步修饰。
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