Sweet potato (Ipomoea batatas (L.) Lam.) is a highly heterozygous autohexaploid crop with high yield and high anthocyanin content. Purple sweet potato is the main source of anthocyanins, and the mechanism of anthocyanin biosynthesis in storage roots has not been fully revealed. In order to reveal the mechanism of anthocyanin biosynthesis and identify new homologous genes involved in anthocyanin biosynthesis in the storage roots of sweet potato, we used Ningzishu 1 and Jizishu 2 as parents to construct a F1 hybrid population. Seven anthocyanin-containing lines and three anthocyanin-free lines were selected for full-length and second-generation transcriptome analyses. A total of 598,375 circular consensus sequencing reads were identified from full-length transcriptome sequencing. After analysis and correction of second-generation transcriptome data, 41,356 transcripts and 18,176 unigenes were obtained. Through a comparative analysis between anthocyanin-containing and anthocyanin-free groups 2329 unigenes were found to be significantly differentially expressed, of which 1235 were significantly up-regulated and 1094 were significantly down-regulated. GO enrichment analysis showed that the differentially expressed unigenes were significantly enriched in molecular function and biological process. KEGG enrichment analysis showed that the up-regulated unigenes were significantly enriched in the flavonoid biosynthesis and phenylpropanoid biosynthesis pathways, and the down-regulated unigenes were significantly enriched in the plant hormone signal transduction pathway. Weighted gene co-expression network analysis of differentially expressed unigenes revealed that anthocyanin biosynthesis genes were co-expressed with transcription factors such as MYB, bHLH and WRKY at the transcription level. Our study will shed light on the regulatory mechanism of anthocyanin biosynthesis in sweet potato storage roots at the transcriptome level.

中文翻译：

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基于杂种群体的全长转录组的比较分析揭示了甘薯（番薯（Ipomoea batatas（L.）Lam））中花色苷生物合成的调控机制。

甘薯（Ipomoea batatas（L.）Lam。）是一种高度杂合的自身六倍体作物，产量高，花色苷含量高。紫色甘薯是花色苷的主要来源，尚未完全揭示花色苷在贮藏根中的生物合成机理。为了揭示花色苷生物合成的机理并在甘薯贮藏根中鉴定出与花色苷生物合成有关的新同源基因，我们以宁自薯1号和继子薯2号为亲本构建了F1杂种群体。选择了七个含花色苷的品系和三个无花色苷的品系用于全长和第二代转录组分析。从全长转录组测序中鉴定出总共598,375个环状共有序列测序读数。经过分析和校正第二代转录组数据后，获得了41,356个转录本和18,176个单基因。通过对含花色素苷和不含花色素苷的组的比较分析，发现2329个单基因显着差异表达，其中1235个显着上调，而1094个显着下调。GO富集分析表明差异表达的单基因在分子功能和生物学过程中显着富集。KEGG富集分析表明，上调的单基因在类黄酮生物合成和苯丙烷类生物合成途径中显着富集，而下调的单基因在植物激素信号转导途径中显着富集。差异表达的单基因的加权基因共表达网络分析表明，花青素生物合成基因在转录水平上与转录因子如MYB，bHLH和WRKY共表达。我们的研究将在转录组水平上阐明甘薯贮藏根中花色苷生物合成的调控机制。