Cancer survivorship has increased considerably, but common cancer treatments may threaten female reproductive health and fertility. In females, standard fertility preservation techniques include egg and embryo banking and ovarian tissue cryopreservation, but these methods are not suitable for all individuals. Emerging fertility preservation technologies include in vitro follicle growth and ovarian bioprosthetics. Although these platforms hold tremendous promise, they remain in the preclinical phase likely because of our inability to adequately phenocopy the complexity of the in vivo ovarian environment. The goal of this study was to use an established research archive of fixed human ovarian tissue established through the Oncofertility Consortium to better understand the dynamics and milieu of growing follicles within the human ovary. We performed a histological analysis of the immediate surroundings of primary and secondary stage follicles. We evaluated oocyte and follicle diameters of these growing follicles, analyzed their growth trajectories, and mapped their precise relationships to other stage follicles within a defined area. We also stratified our findings according to participant age and previous treatment history. Our results serve as in vivo benchmarks for follicles grown in vitro and provide insight into how follicles should be seeded spatially within bioprosthetic ovaries, potentially improving the efficacy and clinical translation of these emerging technologies.

中文翻译：

---

人类卵巢中生长的卵泡的空间分析，以告知组织工程策略。

癌症的存活率已大大提高，但是常见的癌症治疗方法可能会威胁女性的生殖健康和生育能力。在女性中，标准的生育力保存技术包括卵子和胚胎库以及卵巢组织冷冻保存，但是这些方法并不适合所有个人。新兴的生育保护技术包括体外卵泡生长和卵巢生物修复。尽管这些平台具有巨大的前景，但它们可能仍处于临床前阶段，这可能是由于我们无法充分表型化体内复杂性卵巢环境。这项研究的目的是使用通过癌育力联盟建立的固定的人类卵巢组织研究档案，以更好地了解人类卵巢内卵泡的动态和环境。我们对主要和次要卵泡的周围环境进行了组织学分析。我们评估了这些生长卵泡的卵母细胞和卵泡直径，分析了它们的生长轨迹，并将它们与特定阶段的卵泡的精确关系映射到了定义区域内。我们还根据参与者的年龄和既往治疗史对发现进行了分层。我们的研究结果作为体内基准卵泡生长在体外 并提供有关如何在生物义性卵巢中在空间上播种卵泡的见识，从而有可能提高这些新兴技术的功效和临床翻译。