High-order assembly plays a significant role in the formation of living organisms containing a large number of biomacromolecules and, thus, enlightens the construction of nanomaterials that can load macromolecular payloads at a high efficiency. Herein, by choosing anionic hyaluronic acid (HA) as a model payload, we demonstrated how the electrostatic-interaction-induced high-order assembly can be used to load efficiently biomacromolecules into complex coacervate nanodroplets. The resultant assemblies were primarily composed of HA and cationic chitosan oligosaccharide/dextran (COS/Dex) nanogels and had a controllable structure while also exhibiting biological functionality. HA in the assemblies is capable of targeting CD44-overexpressed tumor cells through CD44-mediated endocytic pathways, which are elucidated herein. Therefore, this study provides a reliable approach for the efficient loading of macromolecular payloads into complex coacervate nanodroplets via electrostatic-attraction-induced high-order assembly.

中文翻译：

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高阶朝着能够靶向癌细胞的基于多糖的复合凝聚层纳米液滴的组装。

高阶组装在包含大量生物大分子的活生物体的形成中起着重要作用，因此，启发了可以高效装载大分子负载的纳米材料的构造。本文中，通过选择阴离子透明质酸（HA）作为模型有效载荷，我们证明了静电相互作用诱导的高阶组装可如何有效地将生物大分子加载到复杂的凝聚纳米液滴中。所得的组件主要由HA和阳离子壳聚糖寡糖/葡聚糖（COS / Dex）纳米凝胶组成，具有可控的结构，同时还具有生物学功能。组件中的HA能够通过CD44介导的内吞途径靶向CD44过表达的肿瘤细胞，本文中对此进行了阐明。因此，