Identification of Novel Adipokines through Proteomic Profiling of Small Extracellular Vesicles Derived from Adipose Tissue.,Journal of Proteome Research

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Identification of Novel Adipokines through Proteomic Profiling of Small Extracellular Vesicles Derived from Adipose Tissue.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2020-06-29 , DOI: 10.1021/acs.jproteome.0c00131
Yan Zhang _{1,

2,

3} , Mei Yu _{1,

2} , Jia Dong _{1,

2,

3} , Yue Wu _{1,

2,

4} , Weidong Tian _{1,

2,

3}

Affiliation

Adipose tissue is regarded as a true endocrine organ that releases adipokines to regulate distant targets. Besides the well-studied secretory adipokines, the adipokines carried by small extracellular vesicles derived from adipose tissue (sEV-AT) have not been completely characterized yet. In this study, we conducted a complementary protein profiling on sEV-AT with label-free quantitative proteomic analysis (project accession: PXD013270). A total of 2607 sEV-AT proteins were identified, among which 328 proteins had been annotated as adipokines. Three undefined adipokine candidates (NPM3, STEAP3, and DAD1) were selected for further validation. These three proteins were expressed in both white and brown adipose tissues and upregulated during adipogenic differentiation in both 3T3-L1 cells and adipose-derived stromal/stem cells (ASCs). Expressions of NPM3 and DAD1 in sEV-AT were significantly decreased in obese subjects compared with lean controls, while obesity could not alter the expression of STEAP3. Our profiling study of the sEV-AT proteins expanded the list of adipokines and highlighted the pivotal role of adipokines specifically carried by sEVs in the regulation of multiple biological processes within adipose tissue.

中文翻译：

通过源自脂肪组织的小细胞外囊泡的蛋白质组学分析鉴定新的脂肪因子。

脂肪组织被认为是一种真正的内分泌器官，它可以释放脂肪因子来调节远处的目标。除了经过充分研究的分泌性脂肪因子外，由源自脂肪组织的小细胞外囊泡 (sEV-AT) 携带的脂肪因子尚未完全表征。在这项研究中，我们通过无标记定量蛋白质组学分析对 sEV-AT 进行了互补蛋白质分析（项目编号：PXD013270）。共鉴定出2607个sEV-AT蛋白，其中328个蛋白被注释为脂肪因子。选择了三个未定义的脂肪因子候选者（NPM3、STEAP3 和 DAD1）进行进一步验证。这三种蛋白质在白色和棕色脂肪组织中均有表达，并在 3T3-L1 细胞和脂肪来源的基质/干细胞 (ASC) 的脂肪形成分化过程中上调。与瘦对照相比，肥胖受试者的 sEV-AT 中 NPM3 和 DAD1 的表达显着降低，而肥胖不能改变 STEAP3 的表达。我们对 sEV-AT 蛋白的分析研究扩展了脂肪因子的列表，并强调了 sEV 特异性携带的脂肪因子在调节脂肪组织内多个生物过程中的关键作用。

更新日期：2020-08-08

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