The objective of this research was to develop new technology for possible noncontact, nondestructive, and culture-independent rapid detection of Salmonella using ferromagnetic nanoparticles. Light signal changes of particles, cells, and their reaction stages were investigated. Amino-functionalized ferromagnetic nanoparticles (amino-FMNs) were synthesized and modified by glutaraldehyde to crosslink the attachment of specific antibodies to the particles. The nanoparticle complex was used to capture, concentrate, and isolate Salmonella in a culture broth. Signal changes of the four stages of the nanoparticles-amino-glutaraldehyde-antibodies-Salmonella cell attachments were tracked with sensitive Synchrotron FTIR spectroscopy (SR-FTIR). The unique peaks from these four steps were identified. Results can be applied to develop a new test method or a new test/universal reader for rapid, nondestructive, and culture-independent detection of Salmonella in food products using IR spectroscopy at wave numbers 1454 cm^-1, 1542 cm^-1, and 1414 cm^-1, respectively.

中文翻译：

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同步加速器FTIR光揭示了沙门氏菌上生物功能化磁性纳米粒子附着的信号变化。

这项研究的目的是开发一种新的技术，以利用铁磁纳米颗粒对沙门氏菌进行可能的非接触，非破坏性和与培养无关的快速检测。研究了粒子，细胞及其反应阶段的光信号变化。合成了氨基官能化的铁磁纳米颗粒（amino-FMNs），并通过戊二醛对其进行了改性，以使特定抗体与颗粒的结合交联。纳米颗粒复合物用于捕获，浓缩和分离培养液中的沙门氏菌。纳米颗粒-氨基-戊二醛-抗体-沙门氏菌四个阶段的信号变化使用敏感的同步加速器FTIR光谱仪（SR-FTIR）跟踪细胞附着。从这四个步骤中鉴定出独特的峰。结果可用于开发新的测试方法或使用IR光谱法在波数1454厘米一个新的测试/普遍用于食品产品的快速，非破坏性和非培养检测沙门氏菌的读者^-1，1542厘米^-1，和1414 cm ^-1。