Nonlinear optical microscopy allows for rapid high-resolution microscopy with image contrast generated from the intrinsic properties of the sample. Established modalities, such as multiphoton excited fluorescence and second/third-harmonic generation, can be combined with other nonlinear techniques, such as coherent Raman spectroscopy, which typically allow chemical imaging of a single resonant vibrational mode of a sample. Here, we utilize a single ultrafast laser source to obtain broadband coherent Raman spectra on a microscope, together with other nonlinear microscopy approaches on the same instrument. We demonstrate that the coherent Raman modality allows broadband measurement (>1000 cm⁻¹), with high spectral resolution (<5 cm⁻¹), with a rapid spectral acquisition rate (3-12 kHz). This enables Raman hyperspectral imaging of kilo-pixel images at >11 frames per second.

中文翻译：

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具有快速宽带傅里叶变换相干拉曼模态的多模态激光扫描非线性光学显微镜。

非线性光学显微镜可实现快速高分辨率的显微镜，并具有由样品的内在特性产生的图像对比度。已建立的模态（例如多光子激发荧光和第二/三次谐波生成）可以与其他非线性技术（例如相干拉曼光谱法）结合使用，这些技术通常允许对样品的单个共振振动模式进行化学成像。在这里，我们利用单个超快激光源在显微镜上获得宽带相干拉曼光谱，以及在同一台仪器上的其他非线性显微镜方法。我们证明相干拉曼模态可以进行宽带测量（> 1000 cm ^-1），具有高光谱分辨率（<5 cm ^-1），并具有快速的光谱采集速率（3-12 kHz）。这使得每秒> 11帧的千像素图像的拉曼高光谱成像成为可能。