The culture of live pancreatic tissue slices is a powerful tool for the interrogation of physiology and pathology in an in vitro setting that retains near-intact cytoarchitecture. However, current culture conditions for human pancreatic slices (HPSs) have only been tested for short-term applications, which are not permissive for the long-term, longitudinal study of pancreatic endocrine regeneration. Using a culture system designed to mimic the physiological oxygenation of the pancreas, we demonstrate high viability and preserved endocrine and exocrine function in HPS for at least 10 days after sectioning. This extended lifespan allowed us to dynamically lineage trace and quantify the formation of insulin-producing cells in HPS from both non-diabetic and type 2 diabetic donors. This technology is expected to be of great impact for the conduct of real-time regeneration/developmental studies in the human pancreas.

活胰腺组织切片的培养是在体外环境中研究生理学和病理学的有力工具，可保留近乎完整的细胞结构。然而，目前人类胰腺切片（HPS）的培养条件仅针对短期应用进行了测试，这不适用于胰腺内分泌再生的长期纵向研究。使用旨在模拟胰腺生理氧合的培养系统，我们在切片后至少 10 天证明了 HPS 的高活力和保留的内分泌和外分泌功能。这种延长的寿命使我们能够动态谱系追踪和量化来自非糖尿病和 2 型糖尿病供体的 HPS 中胰岛素生成细胞的形成。该技术预计将对人类胰腺的实时再生/发育研究产生重大影响。