Abstract

A sequence of aroyl selenourea ligands (L1–L3) substituted by aniline and their Ru(II) (η⁶-p-cymene) complexes (1-3), [Ru(II) (η⁶-p-cymene) L] (L = monodentate aroyl selenourea ligand) have been synthesized and characterized the composition of the ligands and their metal complexes. The molecular structures of ligand L1 and complex 3 were also confirmed by single XRD crystal method. The single-crystal XRD study showed that aroyl selenourea ligand coordinates with Ru via Se novel neutral monodentate atom. In vitro DNA interaction studies were investigated by Fluorescence and UV-Visible spectroscopic methods which showed that the intercalative mode of binding is in the order of 1 > 2 > 3 with Ru(II) (η⁶-p-cymene) complexes. Spectroscopic methods have been used for measuring the binding affinity of bovine serum albumin to complex. Moreover, the cytotoxic study of complexes (1–3) were evaluated against HeLa S3, A549, and IMR90 cells, resulting in complexes 1 and 2 showed promising cytotoxic activity against HeLa S3 cell with IC₅₀ values of 24 and 26 µM, respectively. Also, the morphological changes of HeLa S3 and A549 cells were confirmed by fluorescence microscope in the presence of complexes 1 and 2 using AO (acridine orange, 200 µM) and EB (ethidium bromide, 100 µM). In addition, the docking results strongly support the protein binding studies of the complexes.

Communicated by Ramaswamy H. Sarma

摘要

一系列被苯胺取代的芳酰基硒脲配体 ( L1–L3)及其 Ru(II) ( η ⁶ - p -cymene) 配合物(1-3) , [Ru(II) ( η ⁶ - p -cymene) L] (L = 单齿芳酰基硒脲配体) 已被合成并表征了配体及其金属配合物的组成。配体L1和配合物3的分子结构也通过单晶XRD晶体方法证实。单晶XRD研究表明，芳酰基硒脲配体通过Se新型中性单齿原子与Ru配位。体外通过荧光和紫外-可见光谱方法研究了 DNA 相互作用，这表明结合的嵌入模式与 Ru(II) ( η ⁶ - p-伞花烃) 复合物的结合顺序为1 > 2 > 3。光谱方法已用于测量牛血清白蛋白与复合物的结合亲和力。此外，针对 HeLa S3、A549 和 IMR90 细胞评估了复合物 ( 1-3)的细胞毒性研究，导致复合物1和2显示出对 HeLa S3 细胞的有希望的细胞毒活性，IC ₅₀值分别为 24 和 26 µM。此外，在复合物1和2存在下，使用 AO（吖啶橙，200 µM）和 EB（溴化乙锭，100 µM）通过荧光显微镜确认了 HeLa S3 和 A549 细胞的形态变化。此外，对接结果强烈支持复合物的蛋白质结合研究。

由 Ramaswamy H. Sarma 交流