Introduction: Persistent infection with high-risk human papillomavirus (HPV) types is associated with high-grade intraepithelial lesions (HSILs) and invasive cervical cancer. The host immune response plays a key role in whether HPV clears or persists. Most studies on local immune response to HPV collect cervical mucus in order to quantify secreted cytokines; however, cells located inside the tissue can release different cytokines associated with HPV infection. Objective: This study compared the cytokine levels in cervical biopsy specimens of women with abnormal colposcopic findings according to the histopathological results: low-grade intraepithelial lesion (LSIL), HSIL, and no intraepithelial lesion (NSIL). Methods: A cross-sectional study enrolling 141 cervical biopsy specimens examined the cytokine profile for interleukin (IL-) 2, IL-4, IL-10, IL-12, IL-17, and IL-23 and interferon-γ, using the Luminex assay/ELISA. Differences in cytokine levels among the cervical lesion groups were assessed using the Kruskal-Wallis test. Results: The 141 specimens included 90 HSILs, 22 LSILs, and 29 NSILs. IL-2 levels were significantly higher in NSIL samples than in LSIL or in HSIL samples (p = 0.0001) and IL-23 levels were significantly higher in NSIL than in HSIL samples (p = 0.003). Conclusions: Our study shows that in samples from the lesion site point, 2 important pro-inflammatory cytokines, IL-2 and IL-23, are downregulated in HPV lesions.
Acta Cytologica

中文翻译：

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宫颈上皮内病变宫颈活检组织中IL-2和IL-23的下调：一项跨领域研究。

简介：持续感染高危型人乳头瘤病毒（HPV）类型与高度上皮内病变（HSIL）和浸润性宫颈癌相关。宿主免疫反应在HPV清除或持续中起关键作用。大多数有关HPV局部免疫反应的研究都收集宫颈粘液以定量分泌的细胞因子。然而，位于组织内部的细胞可以释放与HPV感染相关的不同细胞因子。目的：根据组织病理学结果：低度上皮内病变（LSIL），HSIL和无上皮内病变（NSIL）的组织病理学结果，比较阴道镜检查异常的女性宫颈活检标本中的细胞因子水平。方法：一项横断面研究招募了141名宫颈活检标本，使用Luminex检查了白介素（IL-）2，IL-4，IL-10，IL-12，IL-17和IL-23和干扰素-γ的细胞因子谱。测定/ ELISA。使用Kruskal-Wallis检验评估宫颈病变组之间细胞因子水平的差异。结果： 141个样本包括90个HSIL，22个LSIL和29个NSIL。NSIL样品中的IL-2水平显着高于LSIL或HSIL样品（p = 0.0001），NSIL中的IL-23水平显着高于HSIL样品（p = 0.003）。结论：我们的研究表明，从病变部位取样的HPV病变中有2种重要的促炎细胞因子IL-2和IL-23被下调。
细胞学学报