A protein-based film (PBF), obtained from black soldier fly prepupae proteins, was assessed for its agronomic performance as mulch. PBF was investigated in a potting experiment and compared with Mater-Bi (MB), polyethylene (PE) and bare soil. During the experiment, degraded surface area, weight and thickness of the film, water evaporated from the pot, and the soil microbiological content (SMC) were recorded. In addition, films were buried to assess their biodegradability and impact on SMC. During the mulching process, the PBF showed a significant degradation. In terms of evaporated water, the PBF performed similarly to MB and worse than PE. Regarding SMC, film of any nature caused an increase in the Clostridia spp. and a decrease of total mesophilic aerobic bacteria and fungi contents. When buried, only PBF recorded a faster biodegradability, showing a reduction of surface area, thickness and weight. PBF and MB highlighted a significant increase in contents of Clostridia spp., mesophilic aerobic bacteria and fungi. Our results reported, for the first time, the valorization of black soldier fly (BSF) prepupae proteins as a biodegradable film for mulching purposes. However, further study is needed to reduce the PBF biodegradability and allow it to be used for the most important mulched crops.

中文翻译：

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黑士兵苍蝇制备蛋白的生物塑料薄膜用作覆盖物：初步结果

从黑士兵苍蝇前p的蛋白质获得的基于蛋白质的薄膜（PBF）被评估了其覆盖农艺性能。在盆栽实验中研究了PBF，并将其与Mater-Bi（MB），聚乙烯（PE）和裸土进行了比较。在实验过程中，记录了降解的表面积，膜的重量和厚度，从锅中蒸发的水以及土壤微生物含量（SMC）。另外，将膜掩埋以评估其生物降解性和对SMC的影响。在覆盖过程中，PBF表现出明显的降解。就蒸发水而言，PBF的性能与MB相似，但比PE差。关于SMC，任何性质的膜都会引起梭状芽胞杆菌的增加。并且减少了总的中温需氧细菌和真菌含量。埋葬后，只有PBF记录了更快的生物降解能力，减少了表面积，厚度和重量。PBF和MB强调了梭状芽胞杆菌，嗜温性好氧细菌和真菌的含量显着增加。我们的结果首次报道了黑士兵苍蝇（BSF）pre蛋白的增值作用，以作为覆盖膜的可生物降解膜。但是，需要进行进一步的研究以降低PBF的生物降解能力，并将其用于最重要的覆盖作物。