Vector‐borne and zoonotic infectious diseases are serious public health concerns that affect approximately half of the world's population. In particular, arthropod‐borne viruses (arboviruses) have contributed to more mortality and morbidity worldwide with the emergence of dengue, chikungunya, yellow fever, and Zika virus diseases. The infections have scaled up due to urbanization, globalization, and international mobility. Traditionally, the spread of mosquito‐borne viral diseases to humans was considered a low health priority concern. However, their categorization as emerging infectious diseases and public health emergencies of international concern has heightened the attention given by the government, academia, research, and industry for the development of timely, cost‐efficient, and sustainable solutions. The urgency has increased in the wake of global climate change. The focus on effective interventions includes epidemiological monitoring, vector control measures, molecular diagnostics, vaccines, and environmental determinants. In this review, we discuss the etiology and predisposition of mosquito‐borne viruses that are detrimental to public health and economically damaging when disseminated as epidemics. We focus on the large‐scale virus surveillance methods with special reference to innovations and interventions in molecular detection science and technologies that include viral nucleic acid isolation, polymerase chain reaction (PCR)‐based diagnostics, and high‐throughput sequencing technologies. In addition, we discuss the development of a viral RNA extraction and PCR‐based diagnostic kit (Invirustech) that can extract viral RNA from mosquitoes with verified applications in PCR‐based molecular diagnostics of Pan‐flavivirus.

中文翻译：

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蚊子大规模病毒监测方法的当前趋势

媒介传播和人畜共患传染病是严重的公共卫生问题，影响着世界上大约一半的人口。特别是，随着登革热、基孔肯雅热、黄热病和寨卡病毒病的出现，节肢动物传播的病毒（虫媒病毒）在全世界造成了更多的死亡率和发病率。由于城市化、全球化和国际流动，感染人数增加。传统上，蚊媒病毒疾病向人类传播被认为是低健康优先问题。然而，将它们归类为国际关注的新发传染病和突发公共卫生事件，提高了政府、学术界、研究界和工业界对制定及时、具有成本效益和可持续的解决方案的关注。在全球气候变化之后，紧迫性有所增加。对有效干预措施的关注包括流行病学监测、媒介控制措施、分子诊断、疫苗和环境决定因素。在这篇综述中，我们讨论了蚊媒病毒的病因和易感性，这些病毒在以流行病的形式传播时会损害公共健康并造成经济损失。我们专注于大规模病毒监测方法，特别参考分子检测科学和技术的创新和干预，包括病毒核酸分离、基于聚合酶链反应 (PCR) 的诊断和高通量测序技术。此外，