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Single Iron Site Nanozyme for Ultrasensitive Glucose Detection.
Small ( IF 13.0 ) Pub Date : 2020-06-29 , DOI: 10.1002/smll.202002343 Min Chen 1 , Huang Zhou 1 , Xiaokang Liu 2 , Tongwei Yuan 3 , Wenyu Wang 1 , Chao Zhao 1 , Yafei Zhao 1 , Fangyao Zhou 1 , Xin Wang 1 , Zhenggang Xue 1 , Tao Yao 2 , Can Xiong 1, 4 , Yuen Wu 1
Small ( IF 13.0 ) Pub Date : 2020-06-29 , DOI: 10.1002/smll.202002343 Min Chen 1 , Huang Zhou 1 , Xiaokang Liu 2 , Tongwei Yuan 3 , Wenyu Wang 1 , Chao Zhao 1 , Yafei Zhao 1 , Fangyao Zhou 1 , Xin Wang 1 , Zhenggang Xue 1 , Tao Yao 2 , Can Xiong 1, 4 , Yuen Wu 1
Affiliation
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Nanomaterials with enzyme‐mimicking characteristics have engaged great awareness in various fields owing to their comparative low cost, high stability, and large‐scale preparation. However, the wide application of nanozymes is seriously restricted by the relatively low catalytic activity and poor specificity, primarily because of the inhomogeneous catalytic sites and unclear catalytic mechanisms. Herein, a support‐sacrificed strategy is demonstrated to prepare a single iron site nanozyme (Fe SSN) dispersed on the porous N‐doped carbon. With well‐defined coordination structure and high density of active sites, the Fe SSN performs prominent peroxidase‐like activity by efficiently activating H2O2 into hydroxyl radical (•OH) species. Furthermore, the Fe SSN is applied in colorimetric detection of glucose through a multienzyme biocatalytic cascade platform. Moreover, a low‐cost integrated agarose‐based hydrogel colorimetric biosensor is designed and successfully achieves the visualization evaluation and quantitative detection of glucose. This work expands the application of single‐site catalysts in the fields of nanozyme‐based biosensors and personal biomedical diagnosis.
中文翻译：
用于超灵敏葡萄糖检测的单一铁位点纳米酶。
具有酶模仿特性的纳米材料因其相对较低的成本，较高的稳定性和大规模的制备而在各个领域引起了广泛的关注。然而，纳米酶的广泛应用由于相对较低的催化活性和较差的特异性而受到严重限制，这主要是由于催化位点不均和催化机理不清楚。在此，证明了一种牺牲支持的策略来制备分散在掺氮多孔碳上的单个铁位点纳米酶（Fe SSN）。Fe SSN具有明确的配位结构和高活性位点密度，可通过有效激活H 2 O 2来执行突出的过氧化物酶样活性。变成羟基自由基（•OH）。此外，Fe SSN通过多酶生物催化级联平台用于葡萄糖的比色检测。此外，设计了一种低成本的基于琼脂糖的集成水凝胶比色生物传感器，并成功实现了葡萄糖的可视化评估和定量检测。这项工作扩大了单中心催化剂在基于纳米酶的生物传感器和个人生物医学诊断领域的应用。
更新日期：2020-08-06
中文翻译：
用于超灵敏葡萄糖检测的单一铁位点纳米酶。
具有酶模仿特性的纳米材料因其相对较低的成本，较高的稳定性和大规模的制备而在各个领域引起了广泛的关注。然而，纳米酶的广泛应用由于相对较低的催化活性和较差的特异性而受到严重限制，这主要是由于催化位点不均和催化机理不清楚。在此，证明了一种牺牲支持的策略来制备分散在掺氮多孔碳上的单个铁位点纳米酶（Fe SSN）。Fe SSN具有明确的配位结构和高活性位点密度，可通过有效激活H 2 O 2来执行突出的过氧化物酶样活性。变成羟基自由基（•OH）。此外，Fe SSN通过多酶生物催化级联平台用于葡萄糖的比色检测。此外，设计了一种低成本的基于琼脂糖的集成水凝胶比色生物传感器，并成功实现了葡萄糖的可视化评估和定量检测。这项工作扩大了单中心催化剂在基于纳米酶的生物传感器和个人生物医学诊断领域的应用。
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