Growing evidence suggests that long non‐coding RNAs (lncRNAs) are associated with carcinogenesis. LncRNA small nucleolar RNA host gene 3 (SNHG3) is up‐regulated in various cancers and positively associated with poor prognosis of these cancers. However, the precise role of lncRNA SNHG3 in bladder cancer (Bca) remains unclear. In our research, we first reported that lncRNA SNHG3 was up‐regulated in bladder cancer tissues and positively related to poor clinical prognosis. Moreover, knockdown of lncRNA SNHG3 significantly suppressed the proliferation, migration, invasion and EMT process of Bca cells in vitro and vivo. Mechanistically, we revealed that suppression of SNHG3 evidently enhanced miR‐515‐5p expression and decreased GINS2 expression at posttranscriptional levels. Moreover, SNHG3 positively regulated GINS2 expression by sponging miR‐515‐5p under a competing endogenous RNA (ceRNA) mechanism. To sum up, our study suggested lncRNA SNHG3 acted as a microRNA sponge and an oncogenic role in the progression of bladder cancer.

中文翻译：

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LncRNA SNHG3通过miR-515-5p/GINS2轴促进膀胱癌增殖和转移。

越来越多的证据表明，长链非编码 RNA (lncRNA) 与致癌作用有关。LncRNA 小核仁 RNA 宿主基因 3 (SNHG3) 在各种癌症中上调，并与这些癌症的不良预后呈正相关。然而，lncRNA SNHG3 在膀胱癌（Bca）中的确切作用仍不清楚。在我们的研究中，我们首先报道了 lncRNA SNHG3 在膀胱癌组织中上调并且与临床预后不良呈正相关。此外，lncRNA SNHG3的敲低在体外和体内显着抑制了Bca细胞的增殖、迁移、侵袭和EMT过程。从机制上讲，我们发现抑制 SNHG3 明显增强了 miR-515-5p 的表达并降低了转录后水平的 GINS2 表达。而且，SNHG3 在竞争性内源 RNA (ceRNA) 机制下通过海绵 miR-515-5p 正向调节 GINS2 表达。总而言之，我们的研究表明 lncRNA SNHG3 在膀胱癌的进展中充当 microRNA 海绵和致癌作用。