Differential spectroscopic analysis of size-dependent phycobilisome from Spirulina maxima,Biotechnology and Applied Biochemistry

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Differential spectroscopic analysis of size-dependent phycobilisome from Spirulina maxima
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-06-29 , DOI: 10.1002/bab.1978
Jong Ho Park ₁ , Hyeon Gi Song ₁ , Yeon Kyu Kim ₁ , Hwa Sung Shin ₁

Affiliation

C-phycocyanin (C-Pc), a photosynthetic pigment for use as a fluorescent indicator or in pharmaceutical, food, and cosmetic products, exists in a phycobilisome complex with allophycocyanin (APC), phycoerythrin (PE), and linker polypeptides. This heterogeneity makes it difficult to quantify phycobilisome composition in an ultraviolet–visible (UV–vis) spectrum. In this study, derivative analysis of UV–vis spectra was successfully applied to display the distinct wavelengths at which C-Pc, APC, and PE have maximal peaks. In all samples, C-Pc of the largest portion had a “zero-crossing” first order, APC did not have a zero-crossing first order, and PE did not have first derivative for zero crossing or local minimum from the 500 and 700 nm, respectively. The results show that derivative analyses coupled with signal smoothing can be applied to elucidate the composition of phycobilisome under various conditions including purification and environment.

中文翻译：

极大螺旋藻大小依赖性藻胆体的差示光谱分析

C-藻蓝蛋白 (C-Pc) 是一种用作荧光指示剂或用于药物、食品和化妆品的光合色素，存在于具有别藻蓝蛋白 (APC)、藻红蛋白 (PE) 和接头多肽的藻胆体复合物中。这种异质性使得很难在紫外-可见（UV-vis）光谱中量化藻胆体的组成。在这项研究中，UV-vis 光谱的导数分析被成功应用于显示 C-Pc、APC 和 PE 具有最大峰值的不同波长。在所有样本中，最大部分的 C-Pc 具有“过零”一阶，APC 没有过零一阶，PE 没有过零或 500 和 700 的局部最小值的一阶导数纳米，分别。

更新日期：2020-06-29

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