The extract of Bidens bipinnata L. exhibited wide spectrum of bioactivities owing to the presence of flavonoids. In this study, a purification process was developed to enrich the flavonoid‐rich extract from B. bipinnata L. (BBTF). AB‐8 resin was selected for the purification of total flavonoids. Response surface methodology coupled with Box–Behnken design was employed to optimize the purification condition; it was optimized as pH 5.1, volume of ethanol 80 ml, flow rate of ethanol 1.8 bed volume per hour (BV/h) and concentration of ethanol 76.0%. The total flavonoid content of BBTF was 56.48% under the optimal conditions. The identification of flavonoids in BBTF was conducted using UHPLC–ESI‐Q‐TOF MS. A total of 14 compounds, including 12 flavonoids, were identified in BBTF. Finally, the in vitro antioxidant activities, α‐glucosidase and α‐amylase inhibitory activities of BBTF were comprehensively analyzed by an analytical hierarchy process. The results indicated that it exhibited higher bioactivities than the crude extract. These findings suggested that the optimized process could significantly enhance the purity of flavonoids and their bioactivities. This study showed a comprehensive analysis of a total flavonoid extract of B. bipinnata L. for the first time, which could provide a useful approach for its purification process and quality control as well as bioactivities.

中文翻译：

---

利用响应面法，UHPLC-Q-TOF MS / MS辅助表征并通过层次分析法综合评估其生物活性，利用大孔树脂富集了Bidens bipinnata L.的富含黄酮的提取物。

由于黄酮类化合物的存在，Bidens bipinnata L.的提取物具有广泛的生物活性。在这项研究中，开发了一种纯化方法以富集来自双歧双歧杆菌的富含类黄酮的提取物。L.（BBTF）。选择AB-8树脂纯化总黄酮。响应面分析法与Box–Behnken设计结合使用，以优化纯化条件。将其优化为pH 5.1，乙醇体积80 ml，乙醇流速1.8床体积/小时（BV / h）和乙醇浓度76.0％。在最佳条件下，BBTF的总黄酮含量为56.48％。使用UHPLC-ESI-Q-TOF MS对BBTF中的类黄酮进行了鉴定。在BBTF中共鉴定出14种化合物，包括12种类黄酮。最后，体外抗氧化活性，α-葡萄糖苷酶和α通过层次分析法对BBTF的淀粉酶抑制活性进行了全面分析。结果表明，它具有比粗提物更高的生物活性。这些发现表明，优化的工艺可以显着提高类黄酮的纯度及其生物活性。这项研究首次显示了对双歧双歧总黄酮提取物的全面分析，这可能为其纯化过程和质量控制以及生物活性提供有用的方法。