In this work, we demonstrate a high-performance Field effect transistor-based biosensor (BioFET) on Al functionalized β-Bi₂O₃ nanofibers for highly selective and rapid detection of serotonin. The β-Bi₂O₃ nanofibers were synthesized using electrospinning technique and conductivity was tailored by surface functionalization using Al nanoparticles. Interdigitated silver electrodes deposited over the flexible polyimide (PI) substrate acts as contact terminals and dielectric substrate respectively while Al functionalized β-Bi₂O₃ nanofibers acts as n-type semiconductor channel and a thermally evaporated silver electrode beneath the PI tape as a back-gate terminal. Detailed characterization reveals the formation of β-phase monoclinic, high mobility, 1-D Bi₂O₃ nanofibers, and Al functionalization was validated using elemental composition studies. The fabricated FET exhibited a transconductance of 5.23 μS and mobility of 64.4 cm² V⁻¹s⁻¹ with an Ion/ Ioff ratio ∼10⁶. The BioFET exhibited an excellent sensitivity of 51.64 μA/nM towards serotonin over a wide linear detection range of 10 nM- 1 μM and a limit of detection of 0.29 nM. This can be ascribed to the excellent change in surface potential upon interaction of serotonin molecules with the electroactive surface functionalized multidimensional nanoparticle-nanofiber network and the back-gate electrode-based control of the Debye screening length. The fabricated BioFET exhibits excellent sensitivity, stability and reproducibility with a rapid response time of 0.8 s. Excellent selectivity towards serotonin in the presence of interfering analytes like ascorbic acid (AA), uric acid (UA), Glucose, Na+, K+, Dopamine (DA) is observed owing to the formation of selective binding complexes between the aromatic amine and β-Bi₂O₃. The real-time applicability of the sensor was successfully tested using urine samples as a test matrix thus establishing it as a promising platform for label free detection of biomolecules in point-of care diagnostics.

在这项工作中，我们证明对Al的基于晶体管的高性能场效应生物传感器（BioFET）官能化β-Bi系_2个ö ₃纳米纤维为血清素的高选择性和快速检测。的β-Bi系₂ ö _3个纳米纤维使用静电技术合成和导电性是通过使用纳米颗粒的Al表面官能化定制。沉积在柔性聚酰亚胺互相交叉的银电极（PI）衬底充当接触端子和介电衬底分别同时铝官能化β-Bi系₂ ö ₃纳米纤维充当n型半导体通道，PI胶带下方的热蒸发银电极充当背栅端子。详细的表征揭示了β相单斜晶，高迁移率的1-D Bi ₂ O ₃纳米纤维的形成，并且使用元素组成研究验证了Al的功能化。制成的FET的跨导为5.23μS，迁移率为64.4 cm ² V ^-1 s ^-1，Ion / Ioff比约为^{10 6}。在10 nM-1μM的宽线性检测范围和0.29 nM的检测限范围内，BioFET对5-羟色胺显示出出色的51.64μA/ nM敏感性。这可以归因于5-羟色胺分子与电活性表面功能化的多维纳米颗粒-纳米纤维网络相互作用以及基于德贝屏蔽长度的基于背栅电极的控制相互作用时表面电势的出色变化。制成的BioFET具有出色的灵敏度，稳定性和可重复性，响应时间为0.8 s。在芳香族胺和β-之间形成选择性结合复合物时，在干扰分析物如抗坏血酸（AA），尿酸（UA），葡萄糖，Na +，K +，多巴胺（DA）的存在下，对5-羟色胺具有极好的选择性。 Bi ₂ O₃。使用尿液样本作为测试基质成功测试了传感器的实时适用性，从而将其确立为在即时诊断中无标签检测生物分子的有前途的平台。