Abstract We demonstrate a highly sensitive enzymatic glucose sensor fabricated by screen printing. To enhance the sensitivity of the sensor, the electrode base was first modified by gold nanoparticles (AuNPs) deposition. Glucose oxidase (GOx) immobilization on the electrode was achieved by pyrrole polymerization using chemical doping method. The fabricated sensor was characterized using optical microscope and scanning electron microscope (SEM) and electrochemical experiments. The glucose detection was performed in 0.1 M phosphate buffer saline (PBS, pH 7.4) solution at operating potential of +0.4 V using amperometric i-t curve. The sensor exhibited a high and reproducible sensitivity of 14.453 nA/mM, response time ~25 s, linear range from 1 mM to 10 mM (R2 = 0.9946) and long-term stability up to 30 days in vitro.

中文翻译：

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基于聚吡咯掺杂葡萄糖氧化酶的针状葡萄糖传感器

摘要 我们展示了一种通过丝网印刷制造的高灵敏度酶促葡萄糖传感器。为了提高传感器的灵敏度，电极底座首先通过金纳米颗粒 (AuNPs) 沉积进行了修饰。葡萄糖氧化酶（GOx）固定在电极上是通过吡咯聚合使用化学掺杂方法实现的。使用光学显微镜和扫描电子显微镜（SEM）和电化学实验对制造的传感器进行表征。葡萄糖检测是在 0.1 M 磷酸盐缓冲盐水 (PBS, pH 7.4) 溶液中在 +0.4 V 的操作电位下使用电流分析 it 曲线进行的。该传感器表现出 14.453 nA/mM 的高且可重复的灵敏度，响应时间 ~25 s，线性范围从 1 mM 到 10 mM (R2 = 0.9946)，并且在体外长达 30 天的长期稳定性。